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首页> 外文期刊>Journal of Biomechanics >Cell relaxation after electrodeformation: effect of latrunculin A on cytoskeletal actin
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Cell relaxation after electrodeformation: effect of latrunculin A on cytoskeletal actin

机译：电极形成后的细胞松弛：latrunculin A对细胞骨架肌动蛋白的影响

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摘要

Precise measurement of the mechanical properties of a cell provides useful information about its structural organization and physiological state. It is interesting to understand the effect of individual components on the mechanical properties of the entire cell. In this study, we investigate the influence of the cytoskeletal actin on the viscoelastic properties of a cell. Actin-specific agents, including latrunculin A and jasplakinolide, are used to alter the organization of the cytoskeletal actin. Brassica oleracea protoplasts are treated with the drugs and deformed under an external electric potential. The relaxation processes of single protoplasts after electrodeformation are measured. The data are analyzed by a model-independent spectrum recovery algorithm. Two distinct characteristic time constants are obtained from the relaxation spectra. Treatment with latrunculin A increases both of the relaxation time constants. The longest relaxation times for control, latrunculin A treated, and jasplakinolide treated cells are determined to be 0.28, 1.0, and 0.21 s, respectively. (C) 2004 Elsevier Ltd. All rights reserved.
机译：细胞机械性能的精确测量提供了有关其结构组织和生理状态的有用信息。了解单个组件对整个电池的机械性能的影响很有趣。在这项研究中，我们调查了细胞骨架肌动蛋白对细胞粘弹性的影响。肌动蛋白特异性药物，包括拉特朗库林A和jasplakinolide，用于改变细胞骨架肌动蛋白的组织。用药物处理甘蓝型油菜原生质体，并在外部电势下变形。测量了电形成后单个原生质体的松弛过程。通过与模型无关的频谱恢复算法分析数据。从弛豫谱获得两个不同的特征时间常数。用latrunculin A处理会增加两个松弛时间常数。对照，经latrunculin A处理和经jasplakinolide处理的细胞的最长弛豫时间分别确定为0.28、1.0和0.21 s。 （C）2004 Elsevier Ltd.保留所有权利。

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