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首页> 外文期刊>Journal of Biomechanics >Micropipette aspiration of living cells [Review]
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Micropipette aspiration of living cells [Review]

机译：微量移液器吸取活细胞[评论]

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The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface. Such edges can be tracked in a light microscope to an accuracy of +/- 25 nm and suction pressures as small as 0.1-0.2 pN/mu m(2) can be imposed on the cell. Both soft cells, such as neutrophils and red cells, and more rigid cells, such as chondrocytes and endothelial cells, are studied with this technique. Interpretation of the measurements with basic continuum models leads to Values for a cell's elastic and viscous properties. In particular, neutrophils are found to behave as a liquid drop with a cortical (surface) tension of about 30 pN/mu m and a viscosity on the order of 100 Pa a. On the other hand, chondrocytes and endothelial cells behave as solids with an elastic modulus of the order of 500 pN/mu m(2) (0.5 kPa). (C) 1999 Published by Elsevier Science Ltd. All rights reserved. [References: 34]
机译：用微量移液管吸取来研究活细胞的机械行为，在该吸管中，细胞的表面被吸到一个小的玻璃管中，同时跟踪其表面的前缘。可以在光学显微镜中将这些边缘跟踪到+/- 25 nm的精度，并且可以对细胞施加小至0.1-0.2 pN /μm（2）的抽吸压力。用这种技术研究了软细胞，例如嗜中性粒细胞和红细胞，以及更坚硬的细胞，例如软骨细胞和内皮细胞。用基本连续模型对测量值的解释会得出单元的弹性和粘性特性的值。特别地，发现中性粒细胞表现为具有约30pN /μm的皮质（表面）张力和约100Pa·a的粘度的液滴。另一方面，软骨细胞和内皮细胞表现为固体，其弹性模量约为500 pN /μm（2）（0.5 kPa）。 （C）1999由Elsevier Science Ltd.出版。保留所有权利。 [参考：34]

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