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首页> 外文期刊>Journal of Biomechanics >Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity.
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Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity.

机译：动态细胞拉伸可增加人类成骨细胞增殖和CICP合成，但会降低骨钙素合成和碱性磷酸酶活性。

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摘要

The cell activity of human-bone-derived cell cultures was studied after mechanical stimulation by cyclic strain at a magnitude occurring in physiologically loaded bone tissue. Monolayers of subconfluently grown human-bone-derived cells were stretched in rectangular silicone dishes with cyclic predominantly uniaxial movement along their longitudinal axes. Strain was applied over two days for 30 min per day with a frequency of 1 Hz and a strain magnitude of 1000 microstrain. Cyclic stretching of the cells resulted in an increased proliferation (10-48%) and carboxyterminal collagen type I propeptide release (7-49%) of human-cancellous bone-derived osteoblasts while alkaline phosphatase activity and osteocalcin release were significantly reduced by 9-25 and 5-32%, respectively. These results demonstrate that cyclic strain at physiologic magnitude leads to an increase of osteoblast activities related to matrix production while those activities which are characteristic for the differentiated osteoblast and relevant for matrix mineralization are decreased.
机译：在受到周期性负荷的机械刺激后，研究了人骨衍生细胞培养物的细胞活性，其强度发生在生理负载的骨组织中。将亚汇合生长的人骨衍生细胞的单层细胞在矩形硅树脂皿中拉伸，并沿其纵轴循环主要为单轴运动。在两天内每天以30 Hz的频率施加应变，频率为1 Hz，应变强度为1000微应变。细胞的循环拉伸导致人源性骨源性成骨细胞的增生（10-48％）和羧基端I型胶原原肽释放（7-49％），而碱性磷酸酶活性和骨钙素释放则显着降低了9- 25％和5-32％。这些结果表明，生理量的周期性应变导致与基质产生有关的成骨细胞活性增加，而分化的成骨细胞所特有且与基质矿化有关的那些活性降低。

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