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首页> 外文期刊>Journal of Biomechanics >VISCOELASTIC PROPERTIES OF CULTURED PORCINE AORTIC ENDOTHELIAL CELLS EXPOSED TO SHEAR STRESS
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VISCOELASTIC PROPERTIES OF CULTURED PORCINE AORTIC ENDOTHELIAL CELLS EXPOSED TO SHEAR STRESS

机译：培养的猪主动脉内皮细胞在剪切应力作用下的粘弹性

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The viscoelastic properties of cultured endothelial cells exposed to shear stress were measured by the micropipette technique and analyzed using a standard linear viscoelastic model. Cells from porcine aorta were cultured on glass coverslips. A shear stress of 2 Pa was applied using a parallel-plate Row chamber. After Row exposure, the cells were detached from the coverslips and suspended in culture medium. The micropipette experiment was performed on single cells under an inverted microscope. The desired negative pressure was applied stepwise to the tip of the micropipette by opening a solenoid valve. The deformation process of cells in the micropipette was observed through a TV camera and recorded on a videotape. To obtain the viscoelastic parameters, a half-space model of an endothelial cell was used. The cell was assumed to be a homogeneous and incompressible material, and a standard linear viscoelastic model was employed to account for the viscoelastic response. Cells exposed to shear stress for 6 h became spherical in shape after detachment from the substrate. In the case of a 24 h exposure, about half of the detached cells retained an elongated shape upon detachment, with the others taking on a spherical shape. The elastic constants, as determined based on the model, were approximately two times higher for the elongated cells than for control cells from static culture, no-Row conditions, indicating that the elongated cells became stiffer. Enhanced viscous properties also were observed for the elongated cells. These viscoelastic properties are considered to be closely related to cytoskeletal structure. Spherical cells upon detachment, even those that had been exposed to shear stress for 24 h, did not show such significant changes in viscoelastic mechanical properties. [References: 22]
机译：通过微量移液管技术测量暴露于剪切应力的培养的内皮细胞的粘弹性，并使用标准线性粘弹性模型进行分析。在玻璃盖玻片上培养来自猪主动脉的细胞。使用平行板行室施加2 Pa的剪切应力。在行暴露之后，将细胞从盖玻片上分离并悬浮在培养基中。微量移液器实验是在倒置显微镜下对单个细胞进行的。通过打开电磁阀，将所需负压逐步施加到微量移液器的尖端。通过电视摄像机观察微量移液管中细胞的变形过程，并将其记录在录像带上。为了获得粘弹性参数，使用了内皮细胞的半空间模型。假定该单元是均匀且不可压缩的材料，并使用标准线性粘弹性模型来解释粘弹性响应。从基片上脱离后，暴露于剪切应力下6 h的细胞变成球形。在24小时暴露的情况下，约一半的分离细胞在分离时保持伸长的形状，其他细胞呈球形。根据模型确定的弹性常数，对于细长细胞而言，是静态培养的无行条件下的对照细胞的弹性常数的两倍，这表明细长细胞变得更硬。对于拉长的细胞也观察到增强的粘性。这些粘弹性质被认为与细胞骨架结构密切相关。分离后的球状细胞，即使是暴露于剪切应力下24小时的球状细胞，也没有显示出粘弹性力学性能的显着变化。 [参考：22]

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