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首页> 外文期刊>Journal of Biomechanics >Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures
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Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures

机译：在自由膨胀和动态加载培养物中软骨细胞接种的琼脂糖构建体的时空发育

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摘要

Dynamic deformational loading has been shown to significantly increase the development of material properties of chondrocyteseeded agarose hydrogels, however little is known about the spatial development of the material properties within these constructs. In this study, a technique that combines video microscopy and optimized digital image correlation, was applied to assess the spatial development of material properties in tissue-engineered cartilage constructs cultured in free-swelling and dynamically-loaded conditions (3 h/day, 5 days/week, and maintained in free-swelling conditions when not being loaded) over a 6-week period. Although homogeneous at day 0, both free-swelling and dynamically loaded samples progressively developed stiffer outer edges and a softer central region. The distribution of GAGs and collagens were shown to mimic this profile. These results indicate that although dynamic loading augments the development of bulk properties in these samples, possibly by overcoming some of the diffusion limitation and nutrient transport issues, the overall profile of construct properties in the axial direction remains qualitatively the same as in free-swelling culture conditions. Poisson's ratio of these constructs increased over time in culture with increased fixed charged density contributed by the GAGs, but this increase was significantly less in dynamically loaded samples by day 42. Polarized light microscopy of Picrosirius Red labeled samples, at an angle perpendicular to the direction of loading, suggests that these differences in Poisson's ratio may be due to improved organization of collagen network in the dynamically loaded samples. (c) 2005 Elsevier Ltd. All rights reserved.
机译：动态变形载荷已显示出显着增加了软骨细胞接种的琼脂糖水凝胶的材料性能，但是对于这些构建体中材料性能的空间发展知之甚少。在这项研究中，将视频显微镜和优化的数字图像相关性相结合的技术应用于评估在自由膨胀和动态加载条件下（3小时/天，5天）培养的组织工程软骨构造中材料特性的空间发展/周，并在6周内保持不膨胀的状态（不加载时）。尽管在第0天均匀，但自由膨胀和动态加载的样品都逐渐形成了较硬的外部边缘和较软的中心区域。 GAG和胶原蛋白的分布显示出可模仿此轮廓。这些结果表明，尽管动态加载可以通过克服一些扩散限制和养分运移问题来增强这些样品的整体性质，但在轴向方向上，构造性质的总体分布与自由膨胀培养在质性上相同条件。这些构建物的泊松比随着时间的推移而增加，GAG导致固定电荷密度增加，但是到第42天时动态加载的样品中这种增加显着减少。Picrosirius Red标记的样品的偏振光显微镜，垂直于方向负载的变化表明泊松比的这些差异可能是由于动态加载的样品中胶原网络的组织改善所致。 （c）2005 Elsevier Ltd.保留所有权利。

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