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首页> 外文期刊>Journal of Biomechanics >The effects of refreezing on the viscoelastic and tensile properties of ligaments.
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The effects of refreezing on the viscoelastic and tensile properties of ligaments.

机译：重新冷冻对韧带的粘弹性和拉伸性能的影响。

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Biomechanical testing protocols for ligaments can be extensive and span two or more days. During this time, a specimen may have to undergo more than one cycle of freezing and thawing. Thus, the objective of this study was to evaluate the effects of refreezing on the viscoelastic and tensile properties of ligaments. The femur-medial collateral ligament-tibia complexes (FMTC) from six pairs of rabbit knees were used for this study. Following sacrifice, one leg in each pair was assigned to the fresh group and the FMTC was immediately dissected and prepared for testing. The contralateral knees were fresh-frozen at -20 degrees C for 3 weeks, thawed, dissected and then refrozen for one additional week before being tested as the refrozen group. The cross-sectional area and shape of the medial collateral ligament (MCL) was measured using a laser micrometer system. Stress relaxation and cyclic stress-relaxation tests in uniaxial tension were performed followed by a load to failure test. When the viscoelastic behavior of the MCL was described by the quasi-linear viscoelastic (QLV) theory, no statistically significant differences could be detected for the five constants (A, B, C, tau1, and tau2) between the fresh and refrozen groups (p > or = 0.07) based on our sample size. In addition, the structural properties of the FMTCs and the mechanical properties of the MCLs were also found to be similar between the two groups (p > or = 0.68). These results suggest that careful refreezing of the specimens had little or no effect on the biomechanical properties measured.
机译：韧带的生物力学测试规程可能会很广泛，并且持续两天或更长时间。在这段时间内，标本可能必须经历一个以上的冷冻和解冻循环。因此，本研究的目的是评估重新冷冻对韧带的粘弹性和拉伸性能的影响。本研究使用了六对兔膝的股骨内侧副韧带-胫骨复合物（FMTC）。处死后，将每对中的一只腿分配给新鲜组，并立即解剖FMTC并准备进行测试。将对侧膝盖在-20摄氏度下新鲜冷冻3周，解冻，解剖，然后再冷冻一周，然后再作为冷冻组进行测试。使用激光测微仪系统测量内侧副韧带（MCL）的横截面积和形状。在单轴拉伸中进行应力松弛和循环应力松弛测试，然后进行破坏载荷测试。当用准线性粘弹性（QLV）理论描述MCL的粘弹性行为时，新鲜组和重新冷冻组之间的五个常数（A，B，C，tau1和tau2）没有统计学上的显着差异（ p>或= 0.07）。此外，还发现两组的FMTC的结构性质和MCL的机械性质相似（p>或= 0.68）。这些结果表明，仔细地重新冷冻样品对测得的生物力学性能几乎没有影响。

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