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首页> 外文期刊>Journal of Biomechanics >Modulation of neonatal growth plate development by ex vivo intermittent mechanical stress.
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Modulation of neonatal growth plate development by ex vivo intermittent mechanical stress.

机译：体外间歇性机械应力对新生儿生长板发育的调节。

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Although growth plate response to mechanical stress has been increasingly studied, our understanding of mechanical modulation of neonatal growth plate is incomplete, especially concerning biochemical changes. This study was designed to explore the cellular and biochemical responses of the cranial base growth plate (CBGP) explant upon cyclic loading. The growth plate with subchondral bone was aseptically isolated from each of 24 neonatal rabbits and fixated in an organ culture system. Cyclic loading was applied to growth plate explants at 200mN and 1Hz for 60min (N=12), whereas control explants were immersed in organ culture for 60min without mechanical loading (N=12). Computerized image analysis revealed that cyclic loading induced significantly more proliferating chondrocytes than unloaded controls (p<0.001), as well as significantly higher growth plate height at 856+/-30mum than the unloaded controls at 830+/-36mum (p<0.05). Immunoblotting with monoclonal antibodies (mAb) disclosed that the average mAb binding area for chondroitin sulfate was significantly higher in the loaded specimens than the unloaded controls at (p<0.001). The average mAb binding area for keratan sulfate was also significantly higher in the loaded specimens than the unloaded controls (p<0.01). Biochemical analysis showed that the average total hyaluronan content of loaded specimens at 0.25+/-0.06mug/mug DNA was significantly higher than the unloaded controls at 0.09+/-0.05mug/mug DNA (p<0.01). Taken together, these data suggest that brief doses of cyclic, intermittent forces activate cellular and molecular responses in the CBGP ex vivo. Whether hyaluronan-mediated pathway is involved in the biological responses of growth plate to mechanical loading warrants additional investigations.
机译：尽管越来越多地研究了生长板对机械应力的反应，但我们对新生儿生长板的机械调节的理解还不完整，尤其是在生化变化方面。这项研究旨在探讨循环负荷后颅底生长板（CBGP）外植体的细胞和生化反应。从24只新生兔子中无菌分离出具有软骨下骨的生长板，并固定在器官培养系统中。将循环载荷以200mN和1Hz的频率施加于生长板外植体60分钟（N = 12），而将对照外植体在无机械载荷的情况下浸入器官培养物中60min（N = 12）。计算机图像分析显示，循环加载比未加载的对照组诱导的软骨细胞增殖更多（p <0.001），并且在856 +/- 30mum的生长板高度明显高于在830 +/- 36mum的对照组（p <0.05）。 。用单克隆抗体（mAb）进行的免疫印迹显示，在加载的样本中，硫酸软骨素的平均mAb结合面积显着高于未加载的对照（p <0.001）。硫酸角质素的平均mAb结合面积在加载的样本中也显着高于未加载的对照（p <0.01）。生化分析表明，在0.25 +/- 0.06mug / mug的DNA样品中，透明质酸的平均含量显着高于在0.09 +/- 0.05mug / mug的样品中透明质酸的含量（p <0.01）。综上所述，这些数据表明，短暂剂量的周期性，间歇性作用力可激活离体CBGP中的细胞和分子反应。透明质酸介导的途径是否参与生长板对机械负荷的生物学反应值得进一步研究。

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