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首页> 外文期刊>Journal of Biomechanics >Parallel-plate fluid flow systems for bone cell stimulation.
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Parallel-plate fluid flow systems for bone cell stimulation.

机译：用于刺激骨细胞的平行板流体流动系统。

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摘要

Bone responds to changes in its mechanical environment, but the mechanisms by which it does so are poorly understood. One hypothesis of mechanosensing in bone states that osteocytes can sense the flow of fluid through the canalicular system. To study this in vitro a number of fluid flow devices have been designed in which cells are placed between parallel plates in sealed chambers. Fluid flows through the chambers at controlled rates, most commonly driven by a peristaltic pump. In addition to fluid flow, high pressures have been observed in these chambers, but the effect of this on the cellular responses has generally been ignored or considered irrelevant, something challenged by recent cellular experiments using pressure only. We have, therefore, devised a system in which we can considerably reduce the pressure while maintaining the flow rate to enable study of their effects individually and in combination. As reducing pressure also reduces the risk of leaks in flow chambers, our system is suitable for real-time microscopical experiments. We present details of the new systems and of experiments with osteoblasts to illustrate the effects of fluid flow with and without additional pressure on the translocation of beta-catenin to the nucleus.
机译：骨骼对机械环境的变化做出了反应，但对其机制的了解却很少。骨骼机械感测的一种假设是，骨细胞可以感知通过小管系统的流体流动。为了体外研究，已经设计了许多流体流动装置，其中将细胞放置在密封室中的平行板之间。流体以受控的速率流经腔室，最常见的是由蠕动泵驱动。除了流体流动外，在这些腔室中还观察到了高压，但是这种现象对细胞反应的影响通常被忽略或认为是无关紧要的，这受到最近仅使用压力的细胞实验的挑战。因此，我们设计了一种系统，在该系统中，我们可以在保持流速的同时显着降低压力，从而能够单独或组合研究其作用。由于降低压力还可以减少流动室泄漏的风险，因此我们的系统适用于实时显微实验。我们介绍了新系统和成骨细胞实验的详细信息，以说明在有和没有额外压力的情况下，流体对β-catenin转运至细胞核的影响。

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