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首页> 外文期刊>Journal of Biomechanics >Effects of stress fiber contractility on uniaxial stretch guiding mitosis orientation and stress fiber alignment.
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Effects of stress fiber contractility on uniaxial stretch guiding mitosis orientation and stress fiber alignment.

机译：应力纤维收缩性对单轴拉伸引导有丝分裂方向和应力纤维排列的影响。

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摘要

It has been documented that mitosis orientation (MO) is guided by stress fibers (SFs), which are perpendicular to exogenous cyclic uniaxial stretch. However, the effect of mechanical forces on MO and the mechanism of stretch-induced SFs reorientation are not well elucidated to date. In the present study, we used murine 3T3 fibroblasts as a model, to investigate the effects of uniaxial stretch on SFO and MO utilizing custom-made stretch device. We found that cyclic uniaxial stretch induced both SFs and mitosis directions orienting perpendicularly to the stretch direction. The F-actin and myosin II blockages, which resulted in disoriented SFs and mitosis directions under uniaxial stretch, suggested a high correlation between SFO and MO. Y27632 (10 muM), ML7 (50 muM, or 75 muM), and blebbistatin (50 muM, or 75 muM) treatments resulted in SFO parallel to the principle stretch direction. Upon stimulating and inhibiting the phosphorylation of myosin light chain (p-MLC), we observed a monotonic proportion of SFO to the level of p-MLC. These results suggested that the level of cell contraction is crucial to the response of SFs, either perpendicular or parallel, to the external stretch. Showing the possible role of cell contractility in tuning SFO under external stretch, our experimental data are valuable to understand the predominant factor controlling SFO response to exogenous uniaxial stretch, and thus helpful for improving mechanical models.
机译：有文献证明，有丝分裂方向（MO）由应力纤维（SFs）引导，应力纤维（SFs）垂直于外源性循环单轴拉伸。然而，到目前为止，尚未很好地阐明机械力对MO的影响以及拉伸诱导的SFs重新定向的机制。在本研究中，我们以鼠类3T3成纤维细胞为模型，利用定制的拉伸装置研究单轴拉伸对SFO和MO的影响。我们发现，循环单轴拉伸会诱导SF和有丝分裂方向垂直于拉伸方向。 F-肌动蛋白和肌球蛋白II阻滞导致单轴拉伸下SFs和有丝分裂方向迷失，表明SFO和MO之间存在高度相关性。 Y27632（10μM），ML7（50μM或75μM）和blebbistatin（50μM或75μM）处理导致SFO平行于主拉伸方向。在刺激和抑制肌球蛋白轻链（p-MLC）的磷酸化后，我们观察到SFO与p-MLC水平呈单调比例。这些结果表明细胞收缩的水平对于垂直或平行于外部拉伸的SF的反应至关重要。我们的实验数据显示了细胞收缩能力在外部拉伸下调节SFO的可能作用，对于了解控制SFO对外源单轴拉伸反应的主要因素非常有价值，因此有助于改善力学模型。

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