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首页> 外文期刊>Journal of Biomechanics >Effect of sulfated glycosaminoglycan digestion on the transverse permeability of medial collateral ligament
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Effect of sulfated glycosaminoglycan digestion on the transverse permeability of medial collateral ligament

机译：硫酸糖胺聚糖消化对内侧副韧带横向渗透的影响

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Dermatan and chondroitin sulfate glycosaminoglycans (GAGs) comprise over 90% of the GAG content in ligament. Studies of their mechanical contribution to soft tissues have reported conflicting results. Measuring the transient compressive response and biphasic material parameters of the tissue may elucidate the contributions of GAGs to the viscoelastic response to deformation. The hypotheses of the current study were that digestion of sulfated GAGs would decrease compressive stress and aggregate modulus while increasing the permeability of porcine medial collateral ligament (MCL). Confined compression stress relaxation experiments were carried out on porcine MCL and tissue treated with chondroitinase ABC (ChABC). Results were fit to a biphasic constitutive model to derive permeability and aggregate modulus. Bovine articular cartilage was used as a benchmark tissue to verify that the apparatus provided reliable results. GAG digestion removed up to 88% of sulfated GAGs from the ligament. Removal of sulfated GAGs increased the permeability of porcine MCL nearly 6-fold versus control tissues. Peak stress decreased significantly. Bovine articular cartilage exhibited the typical reduction of GAG content and resultant decreases in stress and modulus and increases in permeability with ChABC digestion. Given the relatively small amount of GAG in ligament ( < 1% of tissue dry weight) and the significant change in peak stress and permeability upon removal of GAGs, sulfated GAGs may play a significant role in maintaining the apposition of collagen fibrils in the transverse direction, thus supporting dynamic compressive loads experienced by the ligament during complex joint motion.
机译：皮肤性皮肤病和硫酸软骨素糖胺聚糖（GAG）占韧带GAG含量的90％以上。关于它们对软组织的机械作用的研究报告了相互矛盾的结果。测量组织的瞬时压缩响应和双相材料参数可以阐明GAG对变形的粘弹性响应的贡献。当前研究的假设是，硫酸化GAG的消化将减少压缩应力和聚集模量，同时增加猪内侧副韧带（MCL）的渗透性。在猪MCL和用软骨素酶ABC（ChABC）处理的组织上进行了有限的压缩应力松弛实验。将结果拟合到双相本构模型以得出渗透率和聚集模量。牛关节软骨用作基准组织，以验证该装置是否提供了可靠的结果。 GAG消化可从韧带中去除多达88％的硫酸化GAG。硫酸化GAG的去除使猪MCL的通透性比对照组织提高了近6倍。峰值应力明显降低。牛关节软骨表现出典型的GAG含量降低，从而导致应力和模量降低，ChABC消化导致通透性增加。考虑到韧带中的GAG相对较少（<组织干重的1％），并且在去除GAG时峰值应力和通透性发生了显着变化，因此硫酸化GAG可能在维持胶原蛋白原纤维的横向附着方面起着重要作用。 ，从而支持韧带在复杂关节运动过程中承受的动态压缩载荷。

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