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首页> 外文期刊>Journal of biomedical materials research, Part A >Effects of functionalization of PLGA-[Asp-PEG]n copolymer surfaces with Arg-Gly-Asp peptides, hydroxyapatite nanoparticles, and BMP-2-derived peptides on cell behavior in vitro
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Effects of functionalization of PLGA-[Asp-PEG]n copolymer surfaces with Arg-Gly-Asp peptides, hydroxyapatite nanoparticles, and BMP-2-derived peptides on cell behavior in vitro

机译：用Arg-Gly-Asp肽，羟基磷灰石纳米颗粒和BMP-2衍生肽对PLGA- [Asp-PEG] n共聚物表面进行功能化对体外细胞行为的影响

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Functionalization of polymer surfaces has been recognized as a valuable tool to improve their properties that significantly influence cellular behaviors, such as adhesion, proliferation, migration, and differentiation. In stem cell-mediated bone tissue engineering, surface multifunctionalization of polymeric scaffolds with cell-adhesive, osteoconductive, and osteoinductive biomolecules is a critical strategy to improve such properties. However, the traditional surface modification techniques such as physical deposition/adsorption, chemical modification, grafting, and plasma techniques have great limitations for immobilization of multiple bioactive molecules due to multistep procedures. Recently, a universal technique based on musselinspired self-polymerization of dopamine is developed for multifunctional coatings in a simple way. In our study, we used this newly developed technique to incorporate three biomolecules, cell adhesion-promoting (K) 16GRGDSPC peptides, osteoconductive hydroxyapatite (HAp) nanoparticles, and osteoinductive bone morphogenetic protein-2-derived P24 peptides, to functionalize poly(lactide-co-glycolide) (PLGA)-[Asp-PEG]n scaffolds, and the effects on biological behaviors of co-cultured rabbit-derived bone marrow stromal cells in vitro were investigated. The results showed (K) 16GRGDSPC, HAp, and P24 could be immobilized onto the scaffolds through predeposition of polydopamine (pDA) ad-layer, and the surface-modified scaffolds were noncytotoxic as well as the virgin scaffold. The pDAassisted codeposition of (K)16GRGDSPC, HAp, and P24 on the scaffold surfaces significantly promoted cell adhesion, proliferation, osteodifferentiation, and mineralization in vitro with synergistic effects. Taken together, the functionalized PLGA- [Asp-PEG]n polymeric scaffolds achieved significantly elevated affinity, osteoconductive and osteoinductive ability, and may be a potentially promising bone graft substitute for bone repair.
机译：聚合物表面的功能化已被认为是改善其性能的重要工具，这些性能会显着影响细胞的行为，例如粘附，增殖，迁移和分化。在干细胞介导的骨组织工程中，具有细胞粘附性，骨传导性和骨诱导性生物分子的聚合物支架的表面多功能化是改善此类特性的关键策略。但是，传统的表面修饰技术，例如物理沉积/吸附，化学修饰，接枝和等离子体技术，由于多步操作，对于固定多个生物活性分子具有很大的局限性。最近，以简单的方式开发了一种基于多巴胺的贻贝自聚合的通用技术，用于多功能涂料。在我们的研究中，我们使用了这项新开发的技术并结合了三种生物分子：促进细胞黏附的（K）16GRGDSPC肽，骨传导性羟基磷灰石（HAp）纳米颗粒和骨诱导性骨形态发生蛋白2衍生的P24肽，以对聚丙交酯进行功能化研究了共乙交酯（PLGA）-[Asp-PEG] n支架，以及共培养兔衍生的骨髓基质细胞对体外生物学行为的影响。结果表明，（K）16GRGDSPC，HAp和P24可通过预先沉积聚多巴胺（pDA）ad-layer固定在支架上，并且表面修饰的支架与原始支架无细胞毒性。支架表面上的（K）16GRGDSPC，HAp和P24的pDA辅助定位可显着促进细胞粘附，增殖，骨分化和矿化，并具有协同作用。综上所述，功能化的PLGA- [Asp-PEG] n聚合物支架获得了显着提高的亲和力，骨传导性和骨诱导能力，并且可能是潜在有希望的骨移植替代骨修复剂。

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