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首页> 外文期刊>Journal of biomedical informatics. >A computerized cellular imaging system for high content analysis in Monastrol suppressor screens.
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A computerized cellular imaging system for high content analysis in Monastrol suppressor screens.

机译：一种计算机化的细胞成像系统，可在Monastrol抑制器屏幕中进行高含量分析。

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摘要

In this paper, we describe a new bioimage informatics system developed for high content screening (HCS) applications with the goal to extract and analyze phenotypic features of hundreds of thousands of mitotic cells simultaneously. The system introduces the algorithm of multi-phenotypic mitotic analysis (MMA) and integrates that with algorithms of correlation analysis and compound clustering used in gene microarray studies. The HCS-MMA system combines different phenotypic information of cellular images obtained from three-channel acquisitions to distinguish and label individual cells at various phases of mitosis. The proposed system can also be used to extract and count the number of cells in each phase in cell-based assay experiments and archive the extracted data into a structured database for more sophisticated statistical and data analysis. To recognize different mitotic phases, binary patterns are set up based on a known biological mitotic spindle model to characterize cellular morphology of actin, microtubules, and DNA. To illustrate its utility, the HCS-MMA system has been applied to screen the quantitative response of 320 different drug compounds in suppressing Monastrol. The results are validated and evaluated by comparing the performance of HCS-MMA with visual analysis, as well as clustering of the drug compounds under evaluation.
机译：在本文中，我们描述了一种为高含量筛选（HCS）应用开发的新型生物图像信息学系统，旨在同时提取和分析成千上万有丝分裂细胞的表型特征。该系统引入了多表型有丝分裂分析（MMA）算法，并将其与基因芯片研究中使用的相关分析和化合物聚类算法相集成。 HCS-MMA系统结合了从三通道采集获得的细胞图像的不同表型信息，以区分和标记处于有丝分裂各个阶段的单个细胞。所提出的系统还可用于在基于细胞的测定实验中提取和计数每个阶段的细胞数量，并将提取的数据存档到结构化的数据库中，以进行更复杂的统计和数据分析。为了识别不同的有丝分裂阶段，基于已知的生物有丝分裂纺锤体模型建立二进制模式，以表征肌动蛋白，微管和DNA的细胞形态。为了说明其实用性，HCS-MMA系统已用于筛选320种不同药物化合物在抑制莫纳斯特罗尔中的定量反应。通过将HCS-MMA的性能与目测分析以及所评估的药物化合物进行聚类比较，可以验证和评估结果。

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