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首页> 外文期刊>Journal of biomedical materials research, Part A >Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion.
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Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion.

机译：在体外软骨细胞扩增过程中，在连续传代的软骨细胞衍生的细胞外基质上维持软骨基因表达。

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摘要

The loss of cartilaginous phenotype during in vitro expansion culture of chondrocytes is a major barrier for the application of cartilage tissue engineering. The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress chondrocyte phenotype loss. In this study, we developed several types of ECM derived from serially passaged chondrocytes for use as cell-culture substrata and compared their effects on chondrocyte functions. Primary bovine chondrocytes and serially passaged chondrocytes (at passages 2 and 6) were cultured on tissue-culture polystyrene. After culture, the cellular components were selectively removed from the ECM deposited by the cells. The remaining ECM proteins were used as cell-culture substrata. The composition of the deposited ECM depended on the culture stage of the serially passaged chondrocytes used for the ECM production. The deposited ECM supported the adhesion and proliferation of chondrocytes. The effects of the ECM on the chondrocyte dedifferentiation during in vitro passage culture differed dramatically depending on the phenotype of the chondrocytes used to produce the ECM. The primary chondrocyte-derived ECM delayed the chondrocyte dedifferentiation during in vitro passage culture and is a good candidate for chondrocyte subculture for tissue engineering.
机译：软骨细胞体外扩增培养过程中软骨表型的丧失是软骨组织工程应用的主要障碍。预期使用模拟体内细胞外基质（ECM）微环境的基质是抑制软骨细胞表型丧失的有效方法。在这项研究中，我们开发了从连续传代的软骨细胞衍生的几种ECM用作细胞培养基质，并比较了它们对软骨细胞功能的影响。在组织培养聚苯乙烯上培养原代牛软骨细胞和连续传代的软骨细胞（第2和第6代）。培养后，从细胞沉积的ECM中选择性除去细胞成分。其余的ECM蛋白用作细胞培养基质。沉积的ECM的组成取决于用于ECM生产的连续传代的软骨细胞的培养阶段。沉积的ECM支持软骨细胞的粘附和增殖。 ECM对体外传代培养过程中软骨细胞去分化的影响明显不同，这取决于用于产生ECM的软骨细胞的表型。在体外传代培养过程中，原代软骨细胞衍生的ECM延迟了软骨细胞的去分化，并且是组织工程软骨细胞继代培养的良好候选者。

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