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首页> 外文期刊>Journal of biomedical materials research, Part A >Repair of rat critical size calvarial defect using osteoblast-like and umbilical vein endothelial cells seeded in gelatin/hydroxyapatite scaffolds
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Repair of rat critical size calvarial defect using osteoblast-like and umbilical vein endothelial cells seeded in gelatin/hydroxyapatite scaffolds

机译：明胶/羟磷灰石支架植入成骨样和脐静脉内皮细胞修复大鼠临界颅骨缺损

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摘要

The present study used a previously developed three-dimensional Gelatin/Hydroxyapatite (Gel/HA) homogeneous nanocomposite scaffold with porosity of 82% and interconnecting pores ranging from 300 to 500 m. Cell-seeded scaffolds were used to evaluate bone regeneration of rat critical-size calvarial defect. Totally, 36 male Wistar rats were randomly divided into four experimental groups, including blank defect (defects without any graft), blank scaffold (defects filled with Gel/HA scaffold without cells), and two groups of cell-seeded scaffolds (defects filled with either Gel/HA scaffold seeded with osteoblast-like and endothelial cells or osteoblast-like cell-seeded constructs). After 1, 4, and 12 weeks of scaffold implantation, rats were sacrificed and the calvaria were harvested for histological, immunohistochemical and histomorphometric analysis. In vitro tests showed that scaffolds were nontoxic to cells and promoted ideal cellular attachment. In vivo investigation on scaffold revealed that blank calvarial defects indicated incomplete tissue coverage and little evidence of bone healing. However, blank scaffold and cell-seeded scaffolds significantly promoted osteoconduction and ostegogenesis. Taken together, pre-seeded Gel/HA nanocomposite scaffold with osteoblasts and endothelial cells presented an effective combination to improve osteogenesis in the engineered bone implant. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1770-1778, 2016.
机译：本研究使用先前开发的三维明胶/羟基磷灰石（Gel / HA）均匀纳米复合材料支架，其孔隙率为82％，互连孔的范围为300至500 m。细胞接种支架用于评估大鼠临界大小颅盖缺损的骨再生。总共将36只Wistar雄性大鼠随机分为四个实验组，包括空白缺损（无任何移植物的缺损），空白支架（装有无细胞的Gel / HA支架的缺损）和两组细胞接种的支架（缺损的缺损）。接种有成骨细胞样和内皮细胞或成骨细胞样细胞的构建体的Gel / HA支架）。支架植入1、4和12周后，处死大鼠并收集颅盖进行组织学，免疫组织化学和组织形态计量学分析。体外测试表明，支架对细胞无毒，可促进理想的细胞附着。对支架的体内研究显示空白颅盖缺损表明组织覆盖不完全，几乎没有骨愈合的证据。然而，空白支架和细胞种植支架显着促进骨传导和成骨作用。综上所述，预接种的带有成骨细胞和内皮细胞的Gel / HA纳米复合材料支架可有效改善工程骨植入物中的成骨作用。 （c）2016 Wiley Periodicals，Inc.J Biomed Mater Res A部分：104A：1770-1778，2016。

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