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首页> 外文期刊>Bioorganic and Medicinal Chemistry Letters >Selection and characterization of human monoclonal antibodies against Abrin by phage display.
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Selection and characterization of human monoclonal antibodies against Abrin by phage display.

机译：通过噬菌体展示选择和鉴定抗Abrin的人单克隆抗体。

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摘要

Abrin is a highly potent and lethal type II ribosome inactivating toxin that may be used as a biological warfare agent. To date, no human anti-Abrin antibodies have yet to be reported. Herein, we describe the selection and characterization of two human monoclonal antibodies, termed E12 and RF12, which are capable of binding native Abrin with high affinity and specificity. Through surface plasmon resonance studies, we have determined the association and dissociation rate constants and the cross-reactivity for both antibodies. In our developed Biacore-based Abrin detection system, the limit of detection of antibodies E12 and RF12 is 35 and 75 ng/mL, respectively. These concentrations are about 5 x 10(4)-fold lower than the extrapolated Abrin human LD(50). In sum, our data demonstrated the power of human antibody phage display libraries and the promise of these antibodies as detection devices for Abrin.
机译：Abrin是一种高度有效且致命的II型核糖体灭活毒素，可以用作生物战剂。迄今为止，还没有人类抗阿布林抗体的报道。在本文中，我们描述了两种人类单克隆抗体（称为E12和RF12）的选择和表征，它们能够以高亲和力和特异性结合天然Abrin。通过表面等离振子共振研究，我们确定了两种抗体的缔合和解离速率常数以及交叉反应性。在我们开发的基于Biacore的Abrin检测系统中，抗体E12和RF12的检测极限分别为35和75 ng / mL。这些浓度比外推的Abrin人类LD（50）低约5 x 10（4）倍。总而言之，我们的数据证明了人类抗体噬菌体展示文库的强大功能以及这些抗体有望作为Abrin的检测装置。

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