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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Optimization of electrical stimulation parameters for enhanced cell proliferation on biomaterial surfaces.
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Optimization of electrical stimulation parameters for enhanced cell proliferation on biomaterial surfaces.

机译：优化电刺激参数以增强生物材料表面上的细胞增殖。

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摘要

From the point of view of biocompatibility of bone analog materials, cell-material interaction is of fundamental importance. In this article, we report the effect of pulse electric field stimulation on cell-material interaction by analyzing cellular functionality and viability. An in-house fabricated pulse electric field setup was used for the application of electric field during cell culture experiments. To optimize voltage/electric field, the first set of exploratory experiments was conducted with varying field strength at fixed frequency, and subsequently, the frequency of the electrical stimulation was varied to study its influence on the proliferation of L929 mouse fibroblast cells on gelatin-coated control disc. Subsequently, L929 cells were cultured on hydroxyapatite (HA) and HA-40 wt % BaTiO composite. Cell-cultured samples were analyzed qualitatively as well as quantitatively using fluorescence microscope and scanning electron microscope. It has been demonstrated that due to the application of electric field during the cell culture experiment, the cell proliferation and the cell spreading on the surface of the biomaterials were enhanced within a narrow window of voltage/frequency of electrical stimulation. At lower field intensities, the energy density is quite low and increases parabolically with field strength. There is no significant increase in the temperature (DeltaT ~10 K) of the medium due to the application of short duration pulse electric field. This led us to believe that electric field with appropriate strength and duration can enhance the cell-material interaction.
机译：从骨类似物材料的生物相容性的角度来看，细胞间的相互作用至关重要。在本文中，我们通过分析细胞功能和生存力，报告了脉冲电场刺激对细胞与物质相互作用的影响。内部制作的脉冲电场装置用于细胞培养实验中的电场施加。为了优化电压/电场，在固定频率下以不同的场强进行了第一组探索性实验，随后改变了电刺激的频率以研究其对明胶涂层的L929小鼠成纤维细胞增殖的影响控制盘。随后，将L929细胞培养在羟基磷灰石（HA）和HA-40wt％BaTiO复合材料上。使用荧光显微镜和扫描电子显微镜对细胞培养的样品进行定性和定量分析。已经证明，由于在细胞培养实验期间施加了电场，在电刺激的电压/频率的狭窄窗口内，细胞增殖和在生物材料表面上的细胞扩散得以增强。在较低的场强下，能量密度非常低，并且随场强呈抛物线状增加。由于施加了短时脉冲电场，介质的温度（DeltaT〜10 K）没有明显增加。这使我们相信具有适当强度和持续时间的电场可以增强细胞与材料的相互作用。

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