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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Exploring cell compatibility of a fibronectin-functionalized physically crosslinked poly(vinyl alcohol) hydrogel.
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Exploring cell compatibility of a fibronectin-functionalized physically crosslinked poly(vinyl alcohol) hydrogel.

机译：探索纤连蛋白功能化的物理交联聚乙烯醇水凝胶的细胞相容性。

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摘要

Physically crosslinked poly(vinyl alcohol) (PVA) hydrogels prepared using a low-temperature thermally cycled process have tunable mechanical properties that fall within the range of soft tissues, including cardiovascular tissue. An approach to render it hemocompatible is by endothelization, but its hydrophilic nature is not conducive to cell adhesion and spreading. We investigated the functionalization reaction of this class of PVA hydrogel with fibronectin (FN) for adhesion and spreading of primary porcine radial artery cells and vascular endothelial cells. These are cells relevant to small-diameter vascular graft development. FN functionalization was achieved using a multistep reaction, but the activation step involving carbonyl diimidazole normally required for chemically crosslinked PVA was found to be unnecessary. The reaction resulted in an increase in the elastic modulus of the PVA hydrogel but is still well within the range of cardiovascular tissue. Confocal microscopy confirmed the adhesion and spreading of both cell types on the PVA-FN surfaces, whereas cells failed to adhere to the PVA control. This is a first step toward an alternative for the realization of a synthetic replacement small-diameter vascular graft.
机译：使用低温热循环过程制备的物理交联的聚乙烯醇（PVA）水凝胶具有可调节的机械性能，该机械性能在包括心血管组织在内的软组织范围内。使它具有血液相容性的方法是通过内皮化，但是其亲水性不利于细胞粘附和扩散。我们研究了这类PVA水凝胶与纤连蛋白（FN）的功能化反应，以粘附和扩散主要猪of动脉细胞和血管内皮细胞。这些是与小直径血管移植物发育相关的细胞。使用多步反应可实现FN官能化，但是发现化学羰基交联的PVA通常需要的涉及羰基二咪唑的活化步骤是不必要的。该反应导致PVA水凝胶的弹性模量增加，但是仍然很好地在心血管组织范围内。共聚焦显微镜证实了两种细胞在PVA-FN表面上的粘附和扩散，而细胞未能粘附到PVA对照上。这是朝着实现合成替代性小直径血管移植物的替代方案迈出的第一步。

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