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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Local mechanical response of cells to the controlled rotation of magnetic nanorods
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Local mechanical response of cells to the controlled rotation of magnetic nanorods

机译:细胞对磁性纳米棒受控旋转的局部机械响应

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摘要

The mechanical response of the cytoplasm was investigated by the intracellular implantation of magnetic nanorods and exposure to low-frequency rotatory magnetic fields. Nanorods (Pt-Ni, similar to 200 nm diameter) fabricated by electrodeposition in templates of porous alumina with lengths of approximately 2 and 5 mu m were inserted into NIH/3T3 fibroblasts and manipulated with a rotational magnetic field. Nanorod rotation was observed only for torques greater than 3.0 x 10(-16) Nm, suggesting a Bingham-type behavior of the cytoplasm. Higher torques produced considerable deformation of the intracellular material. The cell nucleus and cell membrane were significantly deformed by nanorods actuated by 4.5 x 10(-15) Nm torques. Our results demonstrate that nanorods under magnetic fields are an effective tool to mechanically probe the intracellular environment. We envision that our findings may contribute to the noninvasive and direct mechanical characterization of the cytoplasm. (C) 2014 Wiley Periodicals, Inc.
机译:通过胞内植入磁性纳米棒并暴露于低频旋转磁场来研究细胞质的机械反应。通过电沉积在长度约为2和5μm的多孔氧化铝模板中制成的纳米棒(Pt-Ni,直径类似于200 nm)被插入NIH / 3T3成纤维细胞中,并通过旋转磁场进行操作。仅当扭矩大于3.0 x 10(-16)Nm时才观察到纳米棒旋转,表明胞质具有宾厄姆型行为。较高的扭矩使细胞内材料发生相当大的变形。通过4.5 x 10(-15)Nm的扭矩驱动的纳米棒使细胞核和细胞膜显着变形。我们的结果表明,在磁场下的纳米棒是机械探测细胞内环境的有效工具。我们设想我们的发现可能有助于细胞质的非侵入性和直接机械表征。 (C)2014威利期刊公司

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