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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Fabrication of polyHEMA grids by micromolding in capillaries for cell patterning and single-cell arrays
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Fabrication of polyHEMA grids by micromolding in capillaries for cell patterning and single-cell arrays

机译：通过在毛细管中微模制用于细胞图案和单细胞阵列的polyHEMA网格

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Control of cell adhesion and growth by microfabrication technology and surface chemistry is important in an increasing number of applications in biotechnology and medicine. In this study, we developed a method to fabricate (2hydroxyethyl methacrylate) (polyHEMA) grids on glass by micromolding in capillaries (MIMIC). As a non-fouling biomaterial, polyHEMA was used to inhibit the nonspecific bonding of cells, whereas the glass surface provided a cell adhesive background. The polyHEMA chemical barrier was directly obtained using MIMIC without surface modification, and the microchannel networks used for capillarity were easily achieved by reversibly bonding the polydimethylsiloxane (PDMS) mold and the glass. After fabrication of the polyHEMA micropattern, individual cytophilic microwells surrounded by cytophobic sidewalls were presented on the glass surface. The polyHEMA micropattern proved effective in controlling the shape and spreading of cells, and square-shaped mouse osteoblast MC3T3-E1 cells were obtained in microwell arrays after incubation for 3 days. Moreover, the widths of the microwells in this micropattern were optimized for use as single-cell arrays. The proposed method could be a convenient tool in the field of drug screening, stem cell research, and tissue engineering. (C) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 13751380, 2015.
机译：在越来越多的生物技术和医学应用中，通过微细加工技术和表面化学控制细胞粘附和生长非常重要。在这项研究中，我们开发了一种通过在毛细管中进行微成型（MIMIC）在玻璃上制造（甲基丙烯酸2-羟乙酯）（polyHEMA）网格的方法。作为一种防污生物材料，聚HEMA可用于抑制细胞的非特异性结合，而玻璃表面则提供了细胞粘附的背景。使用MIMIC无需表面改性即可直接获得polyHEMA化学屏障，并且通过可逆地结合聚二甲基硅氧烷（PDMS）模具和玻璃可轻松实现用于毛细管作用的微通道网络。在制备了polyHEMA微图案之后，在玻璃表面上呈现出被亲细胞侧壁包围的单个亲细胞微孔。经证明，polyHEMA微模式可有效控制细胞的形状和扩散，孵育3天后，在微孔阵列中获得方形小鼠成骨细胞MC3T3-E1细胞。而且，该微图案中微孔的宽度被优化用作单细胞阵列。所提出的方法可能是药物筛选，干细胞研究和组织工程领域的便捷工具。 （C）2014 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，103B：13751380，2015年。

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