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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system
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Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system

机译：纤维二糖脱氢酶功能化导尿管作为新型抗生物膜系统

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摘要

Urinary catheters expose patients to a high risk of acquiring nosocomial infections. To prevent this risk of infection, cellobiose dehydrogenase (CDH), an antimicrobial enzyme able to use various oligosaccharides as electron donors to produce hydrogen peroxide using oxygen as an electron acceptor, was covalently grafted onto plasma-activated urinary polydimethylsiloxane (PDMS) catheter surfaces. Successful immobilization of CDH on PDMS was confirmed by Fourier transformed-infrared spectrometry and production of H2O2. The CDH functionalized PDMS surfaces reduced the amount of viable Staphylococcus aureus by 60%, total biomass deposited on the surface by 30% and 70% of biofilm formation. The immobilized CDH was relatively stable in artificial urine over 16 days, retaining 20% of its initial activity. The CDH coated PDMS surface did not affect the growth and physiology of HEK 239 and RAW 264,7 mammalian cells. Therefore this new CDH functionalized catheter system shows great potential for solving the current problems associated with urinary catheters. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1448-1456, 2016.
机译：导尿管使患者面临医院感染的高风险。为了防止这种感染风险，将纤维二糖脱氢酶（CDH）（一种能够使用各种寡糖作为电子供体，使用氧气作为电子受体来产生过氧化氢的抗微生物酶）共价移植到血浆活化的尿液聚二甲基硅氧烷（PDMS）导管表面。通过傅里叶变换红外光谱法和过氧化氢的产生，证实了CDH在PDMS上的成功固定。 CDH官能化的PDMS表面减少了60％的活金黄色葡萄球菌数量，使表面上沉积的总生物量减少了30％和70％的生物膜形成。固定的CDH在人造尿液中在16天内相对稳定，保留了其初始活性的20％。 CDH涂层的PDMS表面不影响HEK 239和RAW 264,7哺乳动物细胞的生长和生理。因此，这种新的CDH功能化导管系统显示出解决与导尿管相关的当前问题的巨大潜力。 （c）2015 Wiley Periodicals，Inc. J Biomed Mater Res B部分：Appl Biomater，104B：1448-1456，2016。

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