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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Optimization of silk films as substrate for functional corneal epithelium growth
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Optimization of silk films as substrate for functional corneal epithelium growth

机译：优化丝膜作为功能性角膜上皮生长的基质

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The corneal epithelium is the first cellular barrier to protect the cornea. Thus, functional tissue engineering of the corneal epithelium is a strategy for clinical transplantation. In this study, the optimization of silk films (SFs) as substrates for functional human corneal epithelium growth was investigated with primary human corneal epithelial cells on SFs, poly-D-lysine (PDL) coated SFs, arginine-glycine-aspartic acid (RGD) modified SFs and PDL blended SFs. PDL coated SFs significantly promoted cell adhesion at early phases in comparison to the other study groups, while PDL blended SF significantly promoted cell migration in a wound healing model. All film modifications promoted cell proliferation and viability, and a multi-layered epithelium was achieved in 4 weeks of culture. The epithelia formed were tightly apposed and maintained an intact barrier function against rose bengal dye penetration. The results suggested that a differentiated human corneal epithelium can be established with primary corneal epithelial cells on SFs in vitro, by optimizing SF composition with PDL. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 431-441, 2016.
机译：角膜上皮是保护角膜的第一个细胞屏障。因此，角膜上皮的功能组织工程是临床移植的策略。在这项研究中，以原代人角膜上皮细胞在SFs，聚D-赖氨酸（PDL）包被的SFs，精氨酸-甘氨酸-天冬氨酸（RGD）上研究了作为功能性人角膜上皮生长基质的丝膜（SFs）的优化。 ）修改后的SF和PDL混合SF。与其他研究组相比，PDL包被的SF在早期阶段显着促进了细胞粘附，而PDL混合SF在伤口愈合模型中显着促进了细胞迁移。所有的膜修饰均能促进细胞增殖和生存能力，并且在培养4周后即可形成多层上皮。形成的上皮紧密并列，并保持完整的屏障功能，抵抗玫瑰红染料的渗透。结果表明，通过用PDL优化SF成分，可以在SFs上用原代角膜上皮细胞建立分化的人角膜上皮。 （c）2015 Wiley Periodicals，Inc.J Biomed Mater Res Part B：Appl Biomater，104B：431-441，2016年。

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