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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Reactive capsule formation around soft-tissue implants is related to cell necrosis
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Reactive capsule formation around soft-tissue implants is related to cell necrosis

机译：软组织植入物周围的反应性胶囊形成与细胞坏死有关

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Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1,6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA fragmentation initially displayed a scattered distribution; at later time points they appeared mainly in the outer portion of the enveloping capsule. The reactive capsule was thicker for the smooth surface, and there was a positive correlation between capsule thickness and propidium-iodide-positive cells at earlier implantation periods. The results suggest that the thickness of the reactive capsule is related to the extent of cell necrosis. It is suggested that the major initiator for this cell necrosis is mechanical shear since cell necrosis was found mainly in areas where mechanical shear could be expected.
机译：将具有光滑或光滑表面的低密度聚甲醛盘植入大鼠腹壁，并在1,6或12周后评估组织反应。通过两种不同的方法检测细胞损伤。可以使用荧光显微镜通过在杀死大鼠之前注射碘化丙啶来鉴定具有膜通透性增加的细胞。其次，通过检测DNA片段来验证细胞死亡。在第1周，相当数量的界面细胞被碘化丙啶染色。碘化丙啶阳性细胞也富集在盘的边缘，与表面纹理无关。随着时间的推移，阳性界面细胞的数量明显减少。 DNA片段化的细胞最初显示出分散的分布。在以后的时间点，它们主要出现在包膜的外部。反应性胶囊较厚，表面光滑，在植入初期，胶囊厚度与碘化丙啶阳性细胞呈正相关。结果表明，反应性胶囊的厚度与细胞坏死的程度有关。提示该细胞坏死的主要引发剂是机械剪切，因为细胞坏死主要在可预期机械剪切的区域中发现。

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