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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Shear stress effects on bacterial adhesion, leukocyte adhesion, and leukocyte oxidative capacity on a polyetherurethane
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Shear stress effects on bacterial adhesion, leukocyte adhesion, and leukocyte oxidative capacity on a polyetherurethane

机译：剪切应力对聚醚氨基甲酸乙酯上细菌粘附，白细胞粘附和白细胞氧化能力的影响

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Infection of implanted cardiovascular biomaterials still occurs despite inherent host defense mechanisms. Using a rotating disk system, we investigated Staphylococcus epidermidis and polymorphonuclear leukocyte (PMN) adhesion to a polyetherurethane urea (PEUU-A') under shear stress (0-17.5 dynes/cm~2) for time periods up to 6 h. In addition, the superoxide (SO) release capacity of PMNs after transient exposure to PEUU-A' under shear stress was determined. Bacterial adhesion in phosphate-buffered saline (PBS) showed a linear shear dependence, decreasing with increasing shear stress. Overall adhesion in PBS decreased with time. However, bacterial adhesion in 25 percent human serum was similar for all time points up to 360 min. adhesion was observed at all shear levels, displaying no shear dependence. In contrast, PMN adhesion demonstrated a strong shear dependence similarly for times up to 240 min, decreasing sharply with increasing shear stress. Although PMNs preexposed to shear stress showed a slightly diminished SO release response compared to fresh cells for all stimuli, it was not statistically significant regardless of the stimulus. We conclude that circulating leukocytes are unable to adhere in regions of high shear which may contain adherent bacteria. In addition, exposure to PEUU-A' and shear stress (in the range 0-18 dynes/cm~2) is insufficient to cause a depression in the oxidative response of PMNs.
机译：尽管有固有的宿主防御机制，植入的心血管生物材料仍会发生感染。我们使用旋转盘系统研究了表皮葡萄球菌和多形核白细胞（PMN）在剪切应力（0-17.5 dynes / cm〜2）下对聚醚尿素尿素（PEUU-A'）的粘附长达6小时。此外，测定了在剪切应力作用下短暂暴露于PEUU-A'之后PMNs的超氧化物（SO）释放能力。磷酸盐缓冲盐水（PBS）中的细菌粘附表现出线性剪切依赖性，随剪切应力的增加而降低。 PBS中的总粘附力随时间下降。但是，直到360分钟的所有时间点，在25％的人血清中的细菌粘附情况都是相似的。在所有剪切水平下均观察到粘附力，无剪切依赖性。相比之下，PMN附着力在长达240分钟的时间内表现出强烈的剪切依赖性，随着剪切应力的增加而急剧下降。尽管与所有刺激相比，暴露于剪切应力下的PMN的SO释放响应均略有下降，但无论刺激如何，其统计意义均不显着。我们得出的结论是，循环白细胞无法粘附在可能含有粘附细菌的高剪切区域。另外，暴露于PEUU-A′和剪切应力（范围为0-18达因/ cm 2）不足以引起PMN的氧化反应的降低。

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