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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P(2)O(5) coatings.
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Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P(2)O(5) coatings.

机译：玻璃对骨肉瘤细胞对血浆喷涂羟基磷灰石/ CaO-P（2）O（5）涂层反应的影响的流式细胞仪分析。

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摘要

Multilayered coatings composed of mixtures of hydroxyapatite (HA) and P(2)O(5)-based bioactive glasses offer potential clinical benefits in orthopedic and dental surgery. In this study double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The cells were cultured on the materials and the effects on cell growth were determined using a spectrophometric assay of a mitochondrial enzyme that is active in viable cells. While none of the materials influenced the growth of the MG63 cells, the HOS cells appeared to undergo less proliferation on all the HA materials. Flow cytometry analysis was carried out using rabbit antibodies against osteonectin, osteopontin, bone sialoprotein, fibronectin, and collagen type I to measure the effects of the materials on key cellular functions. The results showed that the materials downregulated the expression of these extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells. Copyright 1999 John Wiley & Sons, Inc.
机译：由羟基磷灰石（HA）和基于P（2）O（5）的生物活性玻璃的混合物组成的多层涂层可在整形外科和牙科手术中提供潜在的临床益处。在这项研究中，制备了双层等离子喷涂涂层，并使用两种人骨肉瘤细胞株MG63和HOS TE85（HOS）在组织培养中评估了生物学反应。在材料上培养细胞，并使用在活细胞中有活性的线粒体酶进行分光光度法测定对细胞生长的影响。尽管没有一种材料影响MG63细胞的生长，但HOS细胞似乎在所有HA材料上的增殖都较少。使用兔抗骨粘连蛋白，骨桥蛋白，骨唾液蛋白，纤连蛋白和I型胶原的抗体进行流式细胞仪分析，以测量材料对关键细胞功能的影响。结果表明，该材料通过MG63细胞下调了这些细胞外基质抗原的表达，而与在塑料表面孵育的相同细胞相比，它们对HOS细胞的影响较小。值得注意的是，在两种细胞系中，玻璃含量较高的复合材料将结缔组织蛋白的产生恢复到与对照细胞中存在的相似的水平。版权所有1999 John Wiley＆Sons，Inc.

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