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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Protein encapsulation within poly(ethylene glycol)-coated nanospheres. II. Controlled release properties.
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Protein encapsulation within poly(ethylene glycol)-coated nanospheres. II. Controlled release properties.

机译：蛋白包封在聚乙二醇包覆的纳米球中。二。控释特性。

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摘要

The development of injectable nanoparticulate "stealth" carriers for protein delivery is a major challenge. The aim of this work was to investigate the possibility of achieving the controlled release of a model protein, human serum albumin (HSA), from poly(ethylene glycol) (PEG)-coated biodegradable nanospheres (mean diameter of about 200 nm) prepared from amphiphilic diblock PEG-poly(lactic acid) (PLA) copolymers. HSA was efficiently incorporated into the nanospheres, reaching loadings as high as 9% (w/w). Results of the in vitro release studies showed that it is possible to control the HSA release by choosing the appropriate nanosphere size, loading, and composition. These results also revealed that, following their release, HSA molecules readsorbed onto the nanospheres surfaces when they were not protected by a PEG coating. We were surprised to observe that in spite of the water uptake of the PLA-PEG nanospheres [11-29% (w/w)], the copolymer did not significantly degrade after a 15-day incubation period. Therefore, we concluded that during this time HSA release from PLA-PEG nanospheres followed a diffusion mechanism where bulk erosion and surface desorption were negligible. Copyright 1999 John Wiley & Sons, Inc.
机译：用于蛋白质递送的可注射纳米颗粒“隐身”载体的开发是主要挑战。这项工作的目的是研究从聚乙二醇制备的生物可降解纳米球（平均直径约200 nm）中实现模型蛋白人血清白蛋白（HSA）受控释放的可能性。两亲性二嵌段PEG-聚乳酸（PLA）共聚物。 HSA有效地掺入了纳米球中，负载量高达9％（w / w）。体外释放研究的结果表明，可以通过选择合适的纳米球尺寸，载量和组成来控制HSA释放。这些结果还表明，释放后，HSA分子在未受到PEG涂层保护的情况下会重新吸收到纳米球表面。我们惊讶地观察到，尽管PLA-PEG纳米球的吸水率[11-29％（w / w）]，但在15天的温育期后，共聚物并未明显降解。因此，我们得出的结论是，在这段时间内，HSA从PLA-PEG纳米球的释放遵循扩散机制，而整体腐蚀和表面脱附可忽略不计。版权所有1999 John Wiley＆Sons，Inc.

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