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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Enhanced growth of human vascular endothelial cells on negative ion (Ag-)-implanted hydrophobic surfaces.
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Enhanced growth of human vascular endothelial cells on negative ion (Ag-)-implanted hydrophobic surfaces.

机译：在植入负离子（Ag-）的疏水表面上增强人类血管内皮细胞的生长。

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Silver negative ions (Ag-) were implanted to an insulator, polystyrene, in a relatively low ion energy ranging from 5 to 30 keV, and in a dose ranging from 10(14) to 6 x 10(16) ions. cm-2. Surfaces of Ag--implanted polystyrene were studied by means of secondary ion mass spectrometry, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and micro-Raman spectroscopy, and contact angle measurement. As a result of Ag- implantation, the polystyrene surfaces underwent degradation, thereby becoming more hydrophilic with increasing dose and ion energy except an ion energy of 30 keV. The Ag- implantation in polystyrene led to enhanced growth of human vascular endothelial cells, which grew to more extent with increased hydrophilicity of Ag--implanted surfaces except an ion energy of 30 keV. Polystyrene surfaces on which Ag- were implanted up to an ion energy of 30 keV caused the same hydrophobic level as polystyrene surface itself. Nevertheless, the Ag--implanted polystyrene showed relatively good biocompatibility different from polystyrene. Such an improvement in cell adhesion may be related to the formation of a graphite-like structure on polystyrene surfaces by a Ag--implanted process. Moreover, upon plating in a high cell density, human vascular endothelial cells survived even on the polystyrene region of Ag--implanted polystyrene for longer than 1.5 months, while the cells did not grow on untreated polystyrene in the same culture conditions. Copyright 1999 John Wiley & Sons, Inc.
机译：将银负离子（Ag-）以5至30 keV的相对较低的离子能量和10（14）至6 x 10（16）的离子范围注入到绝缘子聚苯乙烯中。厘米2。通过二次离子质谱，X射线光电子能谱，傅里叶变换红外光谱，微拉曼光谱和接触角测量等方法研究了Ag植入的聚苯乙烯的表面。由于Ag注入，聚苯乙烯表面发生降解，因此，除30 keV的离子能量外，随着剂量和离子能量的增加，亲水性增强。聚苯乙烯中的Ag注入导致人类血管内皮细胞的生长增强，除30 keV的离子能量外，随着Ag注入表面亲水性的提高，其生长程度更大。注入了30 keV离子能量的Ag-的聚苯乙烯表面引起的疏水性与聚苯乙烯表面本身相同。然而，与聚苯乙烯不同，植入银的聚苯乙烯显示出相对较好的生物相容性。细胞粘附力的这种改善可能与通过Ag注入法在聚苯乙烯表面形成类石墨结构有关。此外，在高细胞密度下铺板后，即使在Ag植入的聚苯乙烯的聚苯乙烯区域上，人类血管内皮细胞也可以存活超过1.5个月，而在相同的培养条件下，这些细胞不会在未经处理的聚苯乙烯上生长。版权所有1999 John Wiley＆Sons，Inc.

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