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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Comparison of proliferation and growth of human keratinocytes on plasma copolymers of acrylic acid/1,7-octadiene and self-assembled monolayers.
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Comparison of proliferation and growth of human keratinocytes on plasma copolymers of acrylic acid/1,7-octadiene and self-assembled monolayers.

机译：人角质形成细胞在丙烯酸/ 1,7-辛二烯和自组装单层血浆共聚物上的增殖和生长比较。

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Human keratinocytes were cultured on plasma copolymers (PCPs), self-assembled monolayers (SAMs), and tissue culture poly(styrene) (TCPS). Plasma copolymerization was used to deposit films with controlled concentrations of carboxylic acid functional groups (<5%). Human keratinocytes were cultured onto these PCP surfaces, TCPS, and collagen I. A hydrocarbon plasma polymer surface was used as the negative control. Keratinocyte attachment was measured at 24 h and cell proliferation and growth at 3 and 7 days using optical microscopy and DNA concentrations. The PCP surfaces were compared with two SAM systems comprising pure acid and pure hydrocarbon functionalities, and pure gold was used as a control surface. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative, forming confluent sheets of keratinocytes. This result was confirmed by DNA assays that suggested the acid PCP surfaces were performing as well as collagen I. Keratinocytes attached well to gold and acid-terminated SAMs but attached poorly to methyl-terminated SAMs. The acid functionality also promoted proliferation and growth of keratinocytes after several days in culture. DNA assays revealed that keratinocyte growth on the acid surface was higher than on collagen I. Copyright 1999 John Wiley & Sons, Inc.
机译：将人角质形成细胞培养在血浆共聚物（PCP），自组装单分子膜（SAMs）和组织培养聚苯乙烯（TCPS）上。等离子体共聚用于沉积具有受控浓度的羧酸官能团（<5％）的薄膜。将人角质形成细胞培养在这些PCP表面，TCPS和胶原蛋白I上。烃等离子聚合物表面用作阴性对照。使用光学显微镜和DNA浓度在24小时测量角质形成细胞附着，并在3和7天测量细胞增殖和生长。将PCP表面与包含纯酸和纯烃官能度的两个SAM系统进行比较，并使用纯金作为对照表面。含有羧酸官能团的PCP表面促进了角质形成细胞的附着。在这些表面上的附着水平与在胶原蛋白I上所见的水平相当，胶原蛋白I是用于培养角质形成细胞的优选基质。培养几天后，细胞附着良好并增殖，形成融合的角质形成细胞片。 DNA分析证实了该结果，该分析表明酸性PCP表面的性能与胶原蛋白I相同。角质形成细胞与金和酸封端的SAM附着良好，而与甲基封端的SAM附着较弱。培养几天后，酸官能团还促进了角质形成细胞的增殖和生长。 DNA分析表明，酸性表面的角质形成细胞生长高于胶原蛋白I。版权所有1999 John Wiley＆Sons，Inc.

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