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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein
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Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein

机译：牛骨形态发生蛋白增强骨髓/羟基磷灰石复合材料的体内成骨潜能

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摘要

A composite of marrow mesenchymal stem cells and porous hydroxyapatite (HA) has in vivo osteogenic potential. To investigate factors enhancing the osteogenic potential of marrow/HA composites, we prepared a bone morphogenetic protein (BMP) fraction from the 4M guanidine extract of bovine bone by heparin-sepharose affinity chromatography. Marrow/HA composites or composites containing marrow mesenchymal stem cells, BMP, and HA (marrow/BMP/HA composites) were implanted subcutaneously in 7-week-old male Fischer rats. BMP/HA composites and HA alone were also implanted. The implants were harvested after 2, 4, or 8 weeks and were prepared for histological and biochemical studies. Histological examination showed obvious de novo bone formation together with active osteoblasts at 2 weeks, as well as more extensive bone formation at 4 and 8 weeks in many pores of the marrow/BMP/HA composites. The marrow/HA composites did not induce bone formation at 2 weeks, but there was moderate bone formation at 4 weeks. At 2 weeks, only marrow/BMP/ HA composites resulted in intensive osteogenic activity, judging from alkaline phosphatase and osteocalcin expression at both the protein and gene levels. These results indicate that the combination of marrow mesenchymal stem cells, porous HA, and BMP synergistically enhances osteogenic potential, and may provide a rational basis for their clinical application, although further in vivo experiment is needed.
机译：骨髓间充质干细胞和多孔羟基磷灰石（HA）的复合材料具有体内成骨的潜力。为了研究增强骨髓/ HA复合材料成骨潜能的因素，我们通过肝素-琼脂糖亲和色谱法从牛骨的4M胍提取物中制备了骨形态发生蛋白（BMP）组分。将骨髓/ HA复合材料或包含骨髓间充质干细胞，BMP和HA的复合材料（骨髓/ BMP / HA复合材料）皮下植入7周大的雄性Fischer大鼠中。还植入了BMP / HA复合材料和单独的HA。 2、4、8周后收获植入物，并准备进行组织学和生化研究。组织学检查显示在2周时有明显的新生骨形成以及活跃的成骨细胞，并且在第4和第8周时在骨髓/ BMP / HA复合材料的许多孔中形成了更广泛的骨形成。骨髓/ HA复合材料在2周时没有诱导骨形成，但在4周时有中等骨形成。从碱性磷酸酶和骨钙素在蛋白质和基因水平上的表达来看，在第2周时，只有骨髓/ BMP / HA复合物导致强烈的成骨活性。这些结果表明，尽管需要进一步的体内实验，但骨髓间充质干细胞，多孔HA和BMP的组合可协同增强成骨潜能，并可能为其临床应用提供合理的依据。

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