Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+.

Caicedo M; Jacobs JJ; Reddy A; Hallab NJ

首页> 外文期刊>Journal of biomedical materials research, Part A >Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+.

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Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+.

机译：金属离子诱导的人类T细胞（Jurkat）的DNA损伤，凋亡和坏死的分析表明，Ni2 +和V3 +的毒性比其他金属更高：Al3 +，Be2 +，Co2 +，Cr3 +，Cu2 +，Fe3 +，Mo5 +，Nb5 +，Zr2 +。

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It remains unclear how metal released from implants affects cells of the immune system and, in particular, cells of the adaptive immune system, that is, T-helper lymphocytes. In this study, we investigated the effects of aluminum, chromium, cobalt, copper, iron, molybdenum, nickel, niobium, vanadium, and zirconium ions at concentrations from 0.05 to 5.0 mM on human CD4+ T lymphocytes. The DNA damage, apoptosis, necrosis, and proliferation responses of a human T-helper lymphocyte (Jurkat) cell line were evaluated to test our hypothesis that some metals will preferentially induce genotoxicity (DNA damage). Our results demonstrated that metal ions did not preferentially induce Jurkat T-lymphocyte DNA damage prior to other forms of toxicity, that is, apoptosis and/or direct necrosis. Nickel and vanadium induced the most DNA damage and were the most apoptotic metals tested, inducing >50% caspase-9 positive T cells at 0.05 mM and 0.1 mM concentrations, respectively. Cobalt and niobium were the most toxic metals, inducing <50% viability at approximately 0.5 mM concentrations. Nickel and vanadium were the only metals to induce DNA damage at nearly the same concentrations that induced >50% apoptosis (i.e., <0.05 mM). All the metals tested induced T-cell apoptosis at a lower dose than that required to affect DNA damage or toxicity, implying that soluble metals released from implants may not be preferentially genotoxic to lymphocytes.

机译：尚不清楚从植入物中释放的金属如何影响免疫系统的细胞，特别是适应性免疫系统的细胞，即T辅助淋巴细胞。在这项研究中，我们研究了浓度为0.05至5.0 mM的铝，铬，钴，铜，铁，钼，镍，铌，钒和锆离子对人CD4 + T淋巴细胞的影响。评估了人类T辅助淋巴细胞（Jurkat）细胞系的DNA损伤，凋亡，坏死和增殖反应，以检验我们的假设：某些金属会优先诱导遗传毒性（DNA损伤）。我们的结果表明，在其他形式的毒性之前，金属离子不会优先诱导Jurkat T淋巴细胞DNA损伤，即凋亡和/或直接坏死。镍和钒诱导的DNA损伤最多，并且是凋亡测试最多的金属，在0.05 mM和0.1 mM的浓度下分别诱导> 50％的caspase-9阳性T细胞。钴和铌是最剧毒的金属，在大约0.5 mM的浓度下诱导出<50％的活力。镍和钒是诱导DNA损伤的唯一金属，其浓度几乎与诱导> 50％细胞凋亡（即<0.05 mM）的浓度相同。所有测试的金属诱导T细胞凋亡的剂量均低于影响DNA损伤或毒性所需的剂量，这表明从植入物中释放的可溶性金属可能不会优先对淋巴细胞产生遗传毒性。

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《Journal of biomedical materials research, Part A》 |2008年第4期|共9页
作者
Caicedo M; Jacobs JJ; Reddy A; Hallab NJ;
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正文语种 eng
中图分类医用一般科学;
关键词
Metals; DNA Damage; Apoptosis; Toxic effect; T-Lymphocytes; 金属; DNA损伤; 脱噬作用; T淋巴细胞;

机译：Metals;DNA Damage;Apoptosis;Toxic effect;T-Lymphocytes;金属;DNA损伤;脱噬作用;T淋巴细胞;

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1. Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+. [J] . Caicedo M, Jacobs JJ, Reddy A, Journal of biomedical materials research, Part A . 2008,第4期

机译：金属离子诱导的人类T细胞（Jurkat）的DNA损伤，凋亡和坏死的分析表明，Ni2 +和V3 +的毒性比其他金属更高：Al3 +，Be2 +，Co2 +，Cr3 +，Cu2 +，Fe3 +，Mo5 +，Nb5 +，Zr2 +。
2. Analysis of metal ion-induced -DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni~(2+) and V~(3+) are more toxic than other metals: Al~(3+), Be~(2+), Co~(2+), Cr~(3+), Cu~(2+), Fe~(3+), Mo~(5+), Nb~(5+), Zr~(2+) [J] . Marco Caicedo, Joshua J. Jacobs, Anand Reddy Journal of biomedical materials research, Part A . 2008,第4期

机译：分析金属离子诱导的人类T细胞中DNA损伤，凋亡和坏死，表明Ni〜（2+）和V〜（3+）的毒性比其他金属高：Al〜（3+）， Be〜（2 +），Co〜（2 +），Cr〜（3 +），Cu〜（2 +），Fe〜（3 +），Mo〜（5 +），Nb〜（5 +），Zr 〜（2+）

Analysis of metal ion-induced DNA damage, apoptosis, and necrosis in human (Jurkat) T-cells demonstrates Ni2+ and V3+ are more toxic than other metals: Al3+, Be2+, Co2+, Cr3+, Cu2+, Fe3+, Mo5+, Nb5+, Zr2+.

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