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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Effects of transforming growth factor beta1 released from biodegradable polymer microparticles on marrow stromal osteoblasts cultured on poly(propylene fumarate) substrates.
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Effects of transforming growth factor beta1 released from biodegradable polymer microparticles on marrow stromal osteoblasts cultured on poly(propylene fumarate) substrates.

机译：从可生物降解的聚合物微粒释放的转化生长因子beta1对在聚富马酸丙二酯底物上培养的骨髓基质成骨细胞的影响。

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Recombinant human transforming growth factor beta1 (TGF-beta1) was incorporated into microparticles of blends of poly(DL-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) to create a delivery vehicle for the growth factor. The entrapment efficiency of TGF-beta1 in the microparticles containing 5% PEG was 40.3 +/- 1.2% for a TGF-beta1 loading density of 6.0 ng/1 mg of microparticles. For the same loading, 17.9 +/- 0.6 and 32.1 +/- 2.5% of the loaded TGF-beta1 was released after 1 and 8 days, respectively, followed by a plateau for the remaining 3 weeks. Rat marrow stromal cells showed a dose response to TGF-beta1 released from the microparticles similar to that of added TGF-beta1, indicating the activity of TGF-beta1 was retained during microparticle fabrication and after TGF-beta1 release. An optimal TGF-beta1 dosage of 1.0 ng/mL was determined through a 3-day dose response study for maximal alkaline phosphatase (ALP) activity. The TGF-beta1 released from the microparticles loaded with 6.0 ng TGF-beta1/1 mg of microparticles for the optimal dosage of TGF-beta1 enhanced the proliferation and osteoblastic differentiation of marrow stromal cells cultured on poly(propylene fumarate) substrates. The cells showed significantly increased total cell number, ALP activity, and osteocalcin production with values reaching 138,700 +/- 3300 cells/cm(2), 22.8 +/- 1.5 x 10(-7) micromol/min/cell, and 15.9 +/- 1.5 x 10(-6) ng/cell, respectively, after 21 days as compared to cells cultured under control conditions without TGF-beta1. These results suggest that controlled release of TGF-beta1 from the PLGA/PEG blend microparticles may find applications in modulating cellular response during bone healing at a skeletal defect site. Copyright 2000 John Wiley & Sons, Inc.
机译：将重组人转化生长因子beta1（TGF-beta1）掺入聚（DL-乳酸-共-乙醇酸）（PLGA）和聚（乙二醇）（PEG）的混合物的微粒中，以创建生长因子的转运载体。对于6.0 ng / 1 mg微粒的TGF-beta1装载密度，含5％PEG的微粒中TGF-beta1的包封效率为40.3 +/- 1.2％。对于相同的负载，分别在1天和8天后释放了17.9 +/- 0.6％和32.1 +/- 2.5％的TGF-beta1，然后在剩下的3周内达到平稳。大鼠骨髓基质细胞显示出对从微粒释放的TGF-β1的剂量反应，类似于添加的TGF-β1的剂量反应，表明在微粒制造过程中和TGF-β1释放后，TGF-β1的活性得以保留。通过3天的剂量反应研究确定最大碱性磷酸酶（ALP）活性的最佳TGF-beta1剂量为1.0 ng / mL。对于最佳剂量的TGF-β1，从装有6.0 ngTGF-β1/ 1 mg微粒的微粒中释放的TGF-β1增强了在聚富马酸丙二酯底物上培养的骨髓基质细胞的增殖和成骨细胞分化。细胞显示总细胞数，ALP活性和骨钙素产量显着增加，分别达到138,700 +/- 3300细胞/cm(2)、22.8 +/- 1.5 x 10（-7）micromol / min /细胞和15.9 +与在没有TGF-beta1的对照条件下培养的细胞相比，在21天后，分别为1.5 x 10（-6）ng /细胞。这些结果表明，从PLGA / PEG共混物微粒中TGF-β1的受控释放可能在调节骨骼缺损部位骨愈合过程中的细胞反应中得到应用。版权所有2000 John Wiley＆Sons，Inc.

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