...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture
【24h】

Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture

机译：人鼻软骨细胞在微载体旋转培养中的β-1整合素表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Beta-1 integrin plays a major role in cell attachment and is believed to be involved in mediating the interactions of chondrocytes with their environment. We previously reported that articular chondrocytes propagated in microcarrier spinner culture proliferated and reexpressed their chondrocytic protein. The goal of the present study was to investigate the expression of #beta#-1 integrin by chondrocytes growing on the surface of microcarriers. Nasal chondrocytes (4 x 10~3/cm~2) were seeded on microcarriers and incubated at 37 deg C, 5 percent CO_2, 60 rpm. Expression of chondrocyte markers and #beta#-1 integrin was determined using reverse transcriptase-polymerase chain reaction and immunocytochemical analyses. Do novo synthesis of sulfate-containing proteoglycans was studied using ~(35)SO_4 incorporation techniques. Like articular chondrocytes propagated in microcarrier spinner culture, nasal chondrocytes expressed high levels of co-n type II mRNA, whereas collagen type I mRNA levels were low. Aggrecan mRNA was detectable and levels of do novo ~(35)SO_4 incorporation were high. Chondrocytes immunostained intensely for collagen type II and keratan sulfate but did not stain for collagen type I. #beta#-1 integrin mRNA levels were high, and the protein was immunolocalized to regions of cell-to-cell or cell-to-microcarrier contact. The fact the chondrocytes expressed high levels of #beta#-1 integrin raises the possibility that this integrin molecule has a role in the maintenance of the chondrocytic phenotype.
机译：Beta-1整合素在细胞附着中起主要作用，并被认为参与介导软骨细胞与其周围环境的相互作用。我们以前曾报道过，在微载体微调培养中繁殖的关节软骨细胞增殖并重新表达了其软骨蛋白。本研究的目的是研究微载体表面生长的软骨细胞表达＃β＃-1整联蛋白的表达。将鼻软骨细胞（4 x 10〜3 / cm〜2）接种在微载体上，并在37摄氏度，5％CO_2、60 rpm下孵育。使用逆转录聚合酶链反应和免疫细胞化学分析确定软骨细胞标志物和＃beta＃-1整联蛋白的表达。使用〜（35）SO_4掺入技术研究了含硫酸盐蛋白聚糖的合成。像在微载体旋转培养中繁殖的关节软骨细胞一样，鼻软骨细胞表达高水平的co-n II型mRNA，而胶原I型mRNA则较低。可以检测到Aggrecan mRNA，并且从头（〜35）SO_4的掺入水平很高。软骨细胞对II型胶原和硫酸角质素进行了强烈的免疫染色，但对I型胶原没有染色。＃beta＃-1整联蛋白mRNA水平很高，并且该蛋白被免疫定位在细胞间或细胞间微载体接触区域。软骨细胞表达高水平的＃beta＃-1整合素的事实增加了这种整合素分子在维持软骨细胞表型中起作用的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号