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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro cytotoxicity and in vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels.
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In vitro cytotoxicity and in vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels.

机译：聚（富马酸丙二醇酯-共-乙二醇）水凝胶的体外细胞毒性和体内生物相容性。

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The in vitro cytotoxicity and in vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) [P(PF-co-EG)] hydrogels were assessed in order to investigate the influence of poly(ethylene glycol) molecular weight and copolymer composition. These materials have application as injectable cardiovascular implants; cytotoxicity due to leachable products, as well as inflammation caused by the biomaterial itself, may ultimately affect the biocompatibility of the implant. We utilized a 7-day in vitro cytotoxicity assay to quantify cell density and cellular proliferation in the presence of copolymer films. The copolymer films exhibited slight to moderate cytotoxicity toward cultured endothelial cells, showing 20-86% viability relative to controls. Cell viability increased with an increasing weight percent of PEG or, to a lesser extent, the molecular weight of PEG. In vivo biocompatibility was assessed using a cage implantation model over a 21-day time period. This system was used to characterize the local cellular and humoral inflammatory response in the surrounding exudate, as well as the size and density of macrophages adherent to the material itself. All copolymer formulations exhibited excellent biocompatibility relative to controls with no significant differences in total leukocyte count among the different formulations. The in vivo inflammatory reaction displayed normal wound healing over 21 days as shown by a progressive decrease in both leukocyte concentration and enzymatic activity. The surface coverage of the copolymer films remained relatively constant from 7 to 21 days. There were no cells larger than 0.003 mm2, which was previously shown to be the threshold value for foreign-body giant cells. These data suggest that P(PF-co-EG) hydrogels have potential for use as injectable biomaterials.
机译：为了研究聚乙二醇分子量和共聚物组成的影响，评估了聚富马酸丙二醇酯-共-乙二醇[P（PF-co-EG）]水凝胶的体外细胞毒性和体内生物相容性。这些材料可用作可注射的心血管植入物。由于可浸出产物引起的细胞毒性以及生物材料本身引起的炎症可能最终影响植入物的生物相容性。我们利用了一个为期7天的体外细胞毒性试验来量化共聚物膜存在下的细胞密度和细胞增殖。共聚物膜对培养的内皮细胞表现出轻微至中等的细胞毒性，相对于对照显示出20-86％的活力。细胞活力随着PEG重量百分比的增加或PEG分子量的增加而增加。使用笼式植入模型在21天的时间内评估了体内生物相容性。该系统用于表征周围渗出液中的局部细胞和体液炎症反应，以及粘附于材料本身的巨噬细胞的大小和密度。相对于对照，所有共聚物制剂均表现出优异的生物相容性，不同制剂之间的总白细胞计数没有显着差异。体内炎症反应在21天中显示出正常的伤口愈合，如白细胞浓度和酶活性的逐渐降低所表明。共聚物膜的表面覆盖在7至21天保持相对恒定。没有大于0.003 mm2的细胞，以前已显示这是异物巨细胞的阈值。这些数据表明P（PF-co-EG）水凝胶具有用作可注射生物材料的潜力。

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