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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers.
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Role of endothelial cell-substrate contact area and fibronectin-receptor affinity in cell adhesion to HEMA/EMA copolymers.

机译：内皮细胞-基质接触面积和纤连蛋白-受体亲和力在细胞对HEMA / EMA共聚物粘附中的作用。

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The objective of this study was to examine the effect of substrate hydrophobicity on cell-substrate contact area and the affinity between adsorbed fibronectin (Fn) and its receptor. Homo- and copolymer films of hydrophobic ethyl methacrylate (EMA) and hydrophilic hydroxyethyl methacrylate (HEMA) were spun-cast onto glass slides. Bovine aortic endothelial cells (BAEC) were plated for 2 h in serum-free medium onto polymers preadsorbed with Fn. Cells were fixed, labeled, and examined by total internal reflection fluorescence microscopy (TIRFM) to determine the topography of the basal surface as a function of distance from the substrate. Phase contrast microscopy was used to examine the total projected area of adherent cells. The cumulative contact area was greatest on cells attached to surfaces prepared from 0% HEMA and lowest on surfaces with the highest HEMA content. An equilibrium adhesion model used these data together with the critical force for detachment and the Fn density (Burmeister et al., J Biomed Mater Res 1996;30:13-22) to determine the affinity between Fn and its receptor and the bond strength. The affinity and force per bond decreased with increasing HEMA content. These results indicate that differences in the strength of endothelial cell adhesion to polymers are influenced by the conformation of the adsorbed adhesion proteins. Copyright 1999 John Wiley & Sons, Inc.
机译：这项研究的目的是检查底物疏水性对细胞-底物接触面积的影响以及吸附的纤连蛋白（Fn）及其受体之间的亲和力。将疏水性甲基丙烯酸乙酯（EMA）和亲水性甲基丙烯酸羟乙酯（HEMA）的均膜和共聚物薄膜纺丝到载玻片上。将牛主动脉内皮细胞（BAEC）在无血清培养基中铺板2小时到Fn预吸附的聚合物上。将细胞固定，标记并通过全内反射荧光显微镜（TIRFM）检查，以确定基底表面的形貌与距基底的距离的函数。相差显微镜用于检查贴壁细胞的总投影面积。在附着到由0％HEMA制备的表面的细胞上，累积接触面积最大，而在具有最高HEMA含量的表面上，累积接触面积最小。平衡粘附模型使用这些数据以及分离的临界力和Fn密度（Burmeister等人，J Biomed Mater Res 1996; 30：13-22）来确定Fn及其受体之间的亲和力和结合强度。亲和力和每个键的力随HEMA含量的增加而降低。这些结果表明，内皮细胞对聚合物的粘附强度的差异受吸附的粘附蛋白的构象影响。版权所有1999 John Wiley＆Sons，Inc.

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