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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Preparation and characterization of novel poEy(&-caprolactone)/biphasic calcium phosphate hybrid composite microspheres
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Preparation and characterization of novel poEy(&-caprolactone)/biphasic calcium phosphate hybrid composite microspheres

机译：新型聚己内酯/双相磷酸钙杂化复合微球的制备与表征

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Desirably porous biodegradable hybrid composite microspheres were fabricated for use in bone graft and bone substitute applications. In this study, novel poly(epsilon-caprolac-tone)/biphasic calcium phosphate (70/30) composite micro-spheres (PCL/BCP MPs) were prepared using the emulsion solvent-evaporation method. Throughout this process, the ammonium bicarbonate (NH_4HCO_3) content was changed to obtain the desired porous structure. However, to maintain the spherical shape, the NH_4HCO3 content should not be higher than 5%. In the optical images of the PCL/BCP MPs, almost all the microparticles had a spherical shape, and the average diameter was about 600 mum. The scanning electron microscopy and cross-sectional optical images showed that the pore density and pore diameter of PCL/BCP MPs increased with increasing initial NH_4HCO_3 concentrations. In the phase-composition analysis of the PCL/BCP MPs, which was characterized by X-ray diffraction and EDS, the two crystals BCP and PCL phases were shown to be miscible in PCL/ BCP MPs. When the degradation of these microspheres was characterized, PCL/BCP MPs-0, PCL/BCP MPs-2, and PCL/BCP MPs-5 were found to display a sustained biodegradability, and the rate of degradation increased at higher initial NH_4HCO3 concentrations. Proliferation of cells on three different sample types was assessed and compared, and based on these results, the PCL/BCP MPs-5 was chosen to study MG-63 osteoblast-cell adhesion, growth, and proliferation. Furthermore, confocal images indicated that the cells effectively adhered, spread, and proliferated on PCL/BCP MPs-5 during a 5-day culture period.
机译：期望将多孔的可生物降解的杂化复合微球制备用于骨移植和骨替代应用。在这项研究中，使用乳液溶剂蒸发法制备了新型聚（ε-己内酯基）/双相磷酸钙（70/30）复合微球（PCL / BCP MPs）。在整个过程中，改变碳酸氢铵（NH_4HCO_3）的含量以获得所需的多孔结构。但是，为了保持球形，NH_4HCO3的含量不应高于5％。在PCL / BCP MP的光学图像中，几乎所有微粒都具有球形形状，并且平均直径为约600μm。扫描电子显微镜和截面光学图像显示，PCL / BCP MPs的孔密度和孔径随初始NH_4HCO_3浓度的增加而增加。在通过X射线衍射和EDS表征的PCL / BCP MP的相组成分析中，显示BCP和PCL这两个晶体相可与PCL / BCP MP混溶。当表征了这些微球的降解特性时，发现PCL / BCP MPs-0，PCL / BCP MPs-2和PCL / BCP MPs-5表现出持续的生物降解性，并且在较高的初始NH_4HCO3浓度下降解速率增加。评估并比较了三种不同样品类型上的细胞增殖，并基于这些结果，选择PCL / BCP MPs-5来研究MG-63成骨细胞粘附，生长和增殖。此外，共聚焦图像表明细胞在5天的培养期间有效粘附，扩散和增殖在PCL / BCP MPs-5上。

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