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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Optically based-indentation technique for acute rat brain tissue slices and thin biomaterials.
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Optically based-indentation technique for acute rat brain tissue slices and thin biomaterials.

机译：基于光学的压痕技术，用于急性大鼠脑组织切片和薄生物材料。

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Currently, micro-indentation testing of soft biological materials is limited in its capability to test over long time scales due to accumulated instrumental drift errors. As a result, there is a paucity of measures for mechanical properties such as the equilibrium modulus. In this study, indentation combined with optical coherence tomography (OCT) was used for mechanical testing of thin tissue slices. OCT was used to measure the surface deformation profiles after placing spherical beads onto submerged test samples. Agarose-based hydrogels at low-concentrations (w/v, 0.3-0.6%) and acute rat brain tissue slices were tested using this technique over a 30-min time window. To establish that tissue slices maintained cell viability, allowable testing times were determined by measuring neuronal death or degeneration as a function of incubation time with Fluor-Jade C (FJC) staining. Since large deformations at equilibrium were measured, displacements of surface beads were compared with finite element elastic contact simulations to predict the equilibrium modulus, mu(infinity) . Values of mu(infinity) for the low-concentration hydrogels ranged from 0.07 to 1.8 kPa, and mu(infinity) for acute rat brain tissue slices was 0.13 +/- 0.04 kPa for the cortex and 0.09 +/- 0.015 kPa for the hippocampus (for Poisson ratio = 0.35). This indentation technique offers a localized, real-time, and high resolution method for long-time scale mechanical testing of very soft materials. This test method may also be adapted for viscoelasticity, for testing of different tissues and biomaterials, and for analyzing changes in internal structures with loading.
机译：当前，由于累积的仪器漂移误差，软生物材料的微压痕测试在长时间范围内的测试能力受到限制。结果，缺乏用于机械性能的措施，例如平衡模量。在这项研究中，压痕结合光学相干断层扫描（OCT）用于薄组织切片的机械测试。将球形珠粒放在浸没的测试样品上后，使用OCT测量表面变形轮廓。使用这种技术在30分钟的时间范围内测试了低浓度（w / v，0.3-0.6％）的琼脂糖基水凝胶和急性大鼠脑组织切片。为了确定组织切片保持细胞活力，通过用Fluor-Jade C（FJC）染色测量神经元死亡或变性作为孵育时间的函数来确定允许的测试时间。由于测量了平衡时的大变形，因此将表面珠的位移与有限元弹性接触模拟进行了比较，以预测平衡模量mu（infinity）。低浓度水凝胶的mu（infinity）值范围为0.07至1.8 kPa，急性大鼠脑组织切片的mu（infinity）值分别为皮质0.13 +/- 0.04 kPa和海马体0.09 +/- 0.015 kPa （对于泊松比= 0.35）。这种压痕技术提供了一种本地化，实时且高分辨率的方法，用于对非常软的材料进行长期的机械测试。该测试方法还可适用于粘弹性，不同组织和生物材料的测试以及分析内部结构随载荷的变化。

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