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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Complete subchondral bone defect regeneration with a tricalcium phosphate collagen implant and osteoinductive growth factors: A randomized controlled study in G?ttingen minipigs
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Complete subchondral bone defect regeneration with a tricalcium phosphate collagen implant and osteoinductive growth factors: A randomized controlled study in G?ttingen minipigs

机译：用磷酸三钙胶原蛋白植入物和骨诱导性生长因子完成软骨下骨缺损的再生：G？ttingen小型猪的随机对照研究

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摘要

The restoration and reconstruction of osseous defects close to the joint, constitutes a challenging field for reconstructive surgery. A dual-layer implant of β-tricalcium phosphate (TCP) and a collagens I/III scaffold was evaluated in a prospective, randomized comparison in a larger animal model. For this purpose, a standardized osteochondral defect was created in the medial facet of the patellar groove in both stifle joints of G?ttingen minipigs. Critical-size osseous defects were either left empty (spontaneous healing; group 1; n = 12) or treated with the two-layer TCP collagen implant (group 2; n = 12). In group 3 (n = 12), additional growth factor mixture (GFM) was supplemented (bone morphogenetic proteins 2, 3, 4, 6, 7, and TGF-β1, 2, 3). Osseous defect regeneration was assessed at 6, 12, and 52 weeks postoperatively (n = 4). Qualitative and quantitative histomorphometric assessment of defect regeneration and bone substitute resorption was conducted by means of light microscopy, fluorescence microscopy, and microradiography. Critical-size defects did not heal spontaneously throughout follow-up (group 1: max. 21.84 ± 2.81% defect area at 52 weeks). The TCP layer of the implant significantly increased the amount of new bone formation with 29.8 ± 9.68% at 6 weeks and 40.09 ± 4.76% at 12 weeks when compared with controls. After 52 weeks, the TCP was almost fully degraded (4.35 ± 3.70%) and the defect was restored with lamellar trabecular bone (31.28 ± 5.02%). Growth factor supplementation resulted in earlier resorption of the TCP implant and faster defect regeneration. The dual-layer TCP collagen implant is suitable to restore subchondral osseous defects. Additional use of GFM increased the resorption of the TCP layer, but did not foster new bone formation.
机译：靠近关节的骨缺损的修复和重建构成了重建手术的挑战领域。在较大的动物模型中，通过前瞻性随机比较评估了β-磷酸三钙（TCP）和胶原蛋白I / III支架的双层植入物。为此，在G？ttingen小型猪的两个窒息关节中，在pa骨沟内侧刻面形成标准的骨软骨缺损。临界大小的骨缺损要么留空（自然愈合;第1组; n = 12），要么用两层TCP胶原蛋白植入物治疗（第2组; n = 12）。在第3组（n = 12）中，补充了其他生长因子混合物（GFM）（骨形态发生蛋白2、3、4、6、7和TGF-β1、2、3）。术后6、12和52周评估骨缺损再生（n = 4）。通过光学显微镜，荧光显微镜和射线照相术对缺损再生和骨替代物吸收进行定性和定量组织形态学评估。在整个随访过程中，临界大小的缺损并未自愈（第1组：52周时最大缺损面积为21.84±2.81％）。与对照组相比，植入物的TCP层显着增加了新骨形成的量，第6周为29.8±9.68％，第12周为40.09±4.76％。 52周后，TCP几乎完全降解（4.35±3.70％），缺损由片状小梁骨修复（31.28±5.02％）。补充生长因子可以使TCP植入物更早地吸收，并加快缺陷的再生。双层TCP胶原蛋白植入物适合于恢复软骨下骨缺损。额外使用GFM可增加TCP层的吸收，但不会促进新骨的形成。

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