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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes
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Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes

机译：胶原来源对纤维状结构和玻璃化胶原膜性能的影响

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Collagen vitrigel membranes are transparent biomaterials characterized by a densely organized, fibrillar nanostructure that show promise in the treatment of corneal injury and disease. In this study, the influence of different type I collagen sources and processing techniques, including acid-solubilized collagen from bovine dermis (Bov), pepsin-solubilized collagen from human fibroblast cell culture (HuCC), and ficin-solubilized collagen from recombinant human collagen expressed in tobacco leaves (rH), on the properties of the vitrigel membranes was evaluated. Postvitrification carbodiimide crosslinking (CX) was also carried out on the vitrigels from each collagen source, forming crosslinked counterparts BovXL, HuCCXL, and rHXL, respectively. Collagen membrane ultrastructure and biomaterial properties were found to rely heavily on both collagen source and crosslinking. Bov and HuCC samples showed a random fibrillar organization of collagen, whereas rH vitrigels showed remarkable regional fibril alignment. After CX, light transmission was enhanced in all groups. Denaturation temperatures after CX increased in all membranes, of which the highest increase was seen in rH (14.71 degrees C), suggesting improved thermal stability of the collagen fibrils in the membranes. Noncrosslinked rH vitrigels may be reinforced through CX to reach levels of mechanical strength and thermal stability comparable to Bov. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 300-307, 2016.
机译：胶原玻璃质凝胶膜是透明的生物材料，其特征在于紧密组织的纤维状纳米结构，在治疗角膜损伤和疾病方面显示出广阔的前景。在这项研究中，不同类型的I胶原蛋白来源和加工技术的影响，包括牛真皮（Bov）的酸增溶性胶原蛋白，人成纤维细胞培养物（HuCC）的胃蛋白酶增溶性胶原蛋白和重组人胶原蛋白的溶纤蛋白胶原蛋白评价了在烟叶（rH）中表达的玻璃化凝胶膜的特性。还对每种胶原来源的玻璃化凝胶进行了玻璃化后碳二亚胺交联（CX），分别形成了交联的对应物BovXL，HuCCXL和rHXL。发现胶原蛋白膜的超微结构和生物材料特性严重依赖于胶原蛋白来源和交联。 Bov和HuCC样品显示胶原蛋白为随机的原纤维组织，而rH玻璃凝胶显示出显着的区域原纤维排列。 CX后，所有组的透光率均得到增强。 CX后所有膜的变性温度均升高，其中rH（14.71摄氏度）升高最高，表明膜中胶原原纤维的热稳定性得到改善。可以通过CX增强非交联的rH玻璃质凝胶，使其达到与Bov相当的机械强度和热稳定性。 （c）2015 Wiley Periodicals，Inc. J Biomed Mater Res B部分：Appl Biomater，104B：300-307，2016年。

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