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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Adsorption of proteins onto poly(ether urethane) with a phosphorylcholine moiety and influence of preadsorbed phospholipid.
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Adsorption of proteins onto poly(ether urethane) with a phosphorylcholine moiety and influence of preadsorbed phospholipid.

机译：蛋白质通过磷酰胆碱部分吸附到聚（醚氨基甲酸酯）上，并影响预吸附的磷脂。

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In a previous report we demonstrated that the blood compatibility of poly(ether urethane) (PEU) was improved by grafting phosphorylcholine (PC) groups on the surface. The improved blood compatibility was indicated by decreased platelet adsorption/activation and reduced thrombin formation at the polymer surface in experiments in which the surfaces were contacted with platelet-rich plasma in vitro. In the present study, we investigated the effect of grafted PC groups at a PEU surface on protein and phospholipid adsorption. Adsorption of human fibrinogen (Fg), human serum albumin (Alb), human high-molecular-weight kininogen (HMWK), and dioleoyl phosphatidylcholine (DOPC) vesicles was measured by ellipsometry. For this purpose, thin PEU films were cast on silicon wafers. The polymer film was photochemically modified with a PC-containing aryl azide. The presence of PC groups on the polymer surface was demonstrated by ESCA (Electron Spectroscopy for Chemical Analysis). The hydrophilicity of the polymer surface increased by the surface modification, as indicated by a decrease of the contact angle from 59 degrees before to 43 degrees after modification. Our data show that the presence of PC groups has little effect on the adsorption of proteins to a PEU surface. The highest adsorption was observed for Fg (0.49 microgram/cm2 on PC-modified PEU and 0.50 microgram/cm2 on PEU), followed by HMWK (0.28 microgram/cm2 on both PC-modified PEU and PEU), and Alb (0.16 microgram/cm2 on PC-modified PEU and 0.18 microgram/cm2 on PEU). Protein adsorption was further studied on a "biomembrane-like" DOPC bilayer formed on hydrophilic silicon. We found no protein adsorption on this DOPC bilayer. The adsorption of small unilamellar DOPC vesicles on the polymer surfaces amounted to about 0.06 microgram/cm2 (corresponding to circa 30% of monolayer coverage) and was similar for PC-modified PEU and PEU. Despite this partial surface coverage, preadsorbed DOPC on the polymer surface diminished the subsequent adsorption of proteins considerably. These results show that the mere presence of phosphorylcholine groups on a PEU surface is insufficient to suppress protein adsorption. The highly ordered structure of natural phospholipid bilayers seems to be required to suppress protein adsorption effectively.
机译：在以前的报告中，我们证明了通过在表面嫁接磷酰胆碱（PC）基团可以改善聚醚氨基甲酸酯（PEU）的血液相容性。在体外使表面与富含血小板的血浆接触的实验中，降低的血小板吸附/活化和减少的凝血酶在聚合物表面的形成表明血液相容性得到了改善。在本研究中，我们调查了PEU表面接枝的PC基团对蛋白质和磷脂吸附的影响。通过椭圆偏振光度法测量人血纤蛋白原（Fg），人血清白蛋白（Alb），人高分子量激肽原（HMWK）和二油酰基磷脂酰胆碱（DOPC）囊泡的吸附。为此，在硅晶片上浇铸薄PEU膜。用含PC的芳基叠氮化物对聚合物膜进行光化学改性。通过ESCA（用于化学分析的电子光谱）证明了聚合物基团上PC基团的存在。聚合物表面的亲水性通过表面改性而增加，如接触角从改性前的59度降低到改性后的43度所表明的。我们的数据表明，PC基团的存在对蛋白质到PEU表面的吸附几乎没有影响。观察到Fg的最高吸附（在PC改性的PEU上为0.49微克/ cm2，在PEU上为0.50微克/ cm2），然后是HMWK（在PC改性的PEU和PEU上均为0.28微克/ cm2）和Alb（0.16微克/ cm2）。经PC修改的PEU上的cm2和PEU上的0.18微克/ cm2）。在亲水硅上形成的“双膜状” DOPC双层膜上进一步研究了蛋白质吸附。我们发现该DOPC双层上没有蛋白质吸附。小单层DOPC囊泡在聚合物表面的吸附量约为0.06微克/平方厘米（约占单层覆盖率的30％），与PC改性的PEU和PEU相似。尽管有部分表面覆盖，但聚合物表面上预先吸附的DOPC大大减少了随后的蛋白质吸附。这些结果表明，PEU表面仅存在磷酸胆碱基团不足以抑制蛋白质吸附。天然磷脂双层的高度有序的结构似乎是有效抑制蛋白质吸附所必需的。

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