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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Macrophage cytokine response to particles and lipopolysaccharide in vitro.
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Macrophage cytokine response to particles and lipopolysaccharide in vitro.

机译：体外巨噬细胞对颗粒和脂多糖的细胞因子反应。

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Several investigators have suggested that biologic molecules adsorbed onto particles may play a key role in determining macrophage response. Adsorbed endotoxins (bacterial debris) may be of particular importance since they are widely present exogenously and endogenously and adhere strongly to many materials. Murine-transformed peritoneal macrophages (IC-21) were used in this in vitro study. Secretions of IL-1 beta, TNF alpha, and IL-6 were used as a measure of macrophage response to micron-range particles of high-density polyethylene and Co-Cr-Mo alloy, with and without adsorbed lipopolysaccharide (LPS) endotoxin. Little cytokine secretion was measured in response to particles (and to polypropylene experimental chambers) cleaned with ethanol and saline and not exposed to LPS. The lack of macrophage response to cleaned particles has been reported by others and may help reconcile conflicting reports in the literature. Cytokine secretion levels were high in all cases if the chambers (with or without particles) were exposed to LPS (and rinsed to minimize nonbound LPS). Secretion patterns were different with particles present and for polymer versus metal particles. Overall, these results suggest that (1) adsorbed molecules on material surfaces strongly affect macrophage response and (2) particle surface chemistry and microstructure affect the concentration and configuration of adsorbed molecules, further influencing particle interaction with macrophage surface receptors. Copyright 2000 John Wiley & Sons, Inc.
机译：几位研究人员提出，吸附在颗粒上的生物分子可能在确定巨噬细胞反应中起关键作用。吸附的内毒素（细菌碎片）可能特别重要，因为它们是外源性和内源性广泛存在的，并牢固地粘附在许多材料上。小鼠转化的腹膜巨噬细胞（IC-21）用于这项体外研究。 IL-1β，TNFα和IL-6的分泌物被用作巨噬细胞对微米级颗粒的高密度聚乙烯和Co-Cr-Mo合金的反应的量度，有和没有吸附脂多糖（LPS）内毒素。响应于用乙醇和盐水清洗且未暴露于LPS的颗粒（和聚丙烯实验室），几乎没有细胞因子分泌被测量。其他人已经报道了巨噬细胞对清洗过的颗粒缺乏反应，这可能有助于调和文献中相互矛盾的报道。如果将腔室（有或没有颗粒）暴露于LPS（并冲洗以最小化未结合的LPS），则在所有情况下细胞因子的分泌水平都很高。对于存在的颗粒以及对于聚合物与金属颗粒，分泌模式是不同的。总体而言，这些结果表明：（1）材料表面上吸附的分子强烈影响巨噬细胞反应，（2）颗粒表面化学和微观结构影响所吸附分子的浓度和构型，从而进一步影响颗粒与巨噬细胞表面受体的相互作用。版权所有2000 John Wiley＆Sons，Inc.

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