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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Residence-time dependent changes in fibrinogen adsorbed to polymeric biomaterials.
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Residence-time dependent changes in fibrinogen adsorbed to polymeric biomaterials.

机译：纤维蛋白原吸附到聚合物生物材料上的停留时间依赖性变化。

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It has generally been accepted that biomaterials adsorbing the least amount of the plasma protein fibrinogen following exposure to blood will support less platelet adhesion and therefore exhibit less thrombogenicity. Several studies suggest, however, that the conformation or orientation of immobilized fibrinogen rather than the total amount adsorbed plays an important role in determining the blood compatibility of biomaterials. The purpose of this study was to investigate time-dependent functional changes in fibrinogen adsorbed to polytetrafluoroethylene (PTFE), polyethylene (PE), and silicone rubber (SR). Fibrinogen was adsorbed to these materials for 1 min and then allowed to 'reside" on the surfaces for up to 2 h prior to assessing its biological activity. Changes in fibrinogen reactivity were determined by measuring the adhesion of 51Cr-labeled platelets, the binding of a monoclonal antibody (mAb) directed against an important functional region of the fibrinogen molecule (the gamma-chain dodecapeptide sequence 400-411), and the ability of blood plasma to displace previously adsorbed fibrinogen. Platelet adhesion differed among the polymeric materials studied, and PTFE and PE samples exhibited a small decrease in adhesion with increasing fibrinogen residence time. Platelet adhesion to SR was the least among all materials studied and showed no variation with residence time. When using PTFE and SR as substrates, mAb recognition of adsorbed fibrinogen did not change with residence time whereas that on PE decreased slightly. The mAb binding was least to fibrinogen adsorbed to SR, which is in agreement with the platelet adhesion results. Finally, the ability of plasma to displace previously adsorbed fibrinogen decreased dramatically with increasing residence time on all materials. These in vitro studies support the hypothesis that fibrinogen undergoes biologically significant conformational changes upon adsorption to polymeric biomaterials, a phenomenon that may contribute to the hemocompatibility of the materials following implantation in the body. Copyright 1999 John Wiley & Sons, Inc.
机译：通常已经接受的是，暴露于血液后吸附最少量血浆蛋白纤维蛋白原的生物材料将支持较少的血小板粘附，因此显示较少的血栓形成性。然而，一些研究表明，固定化纤维蛋白原的构象或方向，而不是吸附的总量，在确定生物材料的血液相容性方面起着重要作用。这项研究的目的是研究纤维蛋白原吸附到聚四氟乙烯（PTFE），聚乙烯（PE）和硅橡胶（SR）上随时间变化的功能变化。在评估其生物活性之前，将纤维蛋白原吸附到这些材料上1分钟，然后使其在表面上“停留” 2小时，然后通过测量51Cr标记的血小板的粘附性，针对纤维蛋白原分子重要功能区域（γ-链十二肽序列400-411）的单克隆抗体（mAb），以及血浆置换先前吸附的纤维蛋白原的能力。 PTFE和PE样品的黏附力随纤维蛋白原停留时间的增加而减小，血小板对SR的黏附力在所有研究的材料中最少，并且随停留时间的变化无变化;当使用PTFE和SR作为底物时，mAb不能识别吸附的纤维蛋白原随停留时间的变化而变化，而在PE上则略有下降，mAb与吸附在SR上的纤维蛋白原的结合最少。与血小板粘附结果一致。最后，随着血浆在所有材料上停留时间的增加，血浆置换先前吸附的纤维蛋白原的能力急剧下降。这些体外研究支持以下假设：纤维蛋白原在吸附到聚合生物材料上时会发生生物学上显着的构象变化，这种现象可能会导致植入体内后材料的血液相容性。版权所有1999 John Wiley＆Sons，Inc.

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