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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro
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Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

机译：脱水的人羊膜/绒毛膜在体外调节干细胞活性

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Human-derived placental tissues have been shown in randomized clinical trials to be effective for healing chronic wounds, and have also demonstrated the ability to recruit stem cells to the wound site in vitro and in vivo. In this study, PURION (R) Processed dehydrated human amnion/chorion membrane allografts (dHACM, EpiFix((R)), MiMedx Group, Marietta, GA) were evaluated for their ability to alter stem cell activity in vitro. Human bone marrow mesenchymal stem cells (BM-MSCs), adipose derived stem cells (ADSCs), and hematopoietic stem cells (HSCs) were treated with soluble extracts of dHACM tissue, and were evaluated for cellular proliferation, migration, and cytokine secretion. Stem cells were analyzed for cell number by DNA assay after 24 h, closure of an acellular zone using microscopy over 3 days, and soluble cytokine production in the medium of treated stem cells was analyzed after 3 days using a multiplex ELISA array. Treatment with soluble extracts of dHACM tissue stimulated BM-MSCs, ADSCs, and HSCs to proliferate with a significant increase in cell number after 24 h. dHACM treatment accelerated closure of an acellular zone by ADSCs and BM-MSCs after 3 days, compared to basal medium. BM-MSCs, ADSCs, and HSCs also modulated endogenous production of a number of various soluble signals, including regulators of inflammation, mitogenesis, and wound healing. dHACM treatment promoted increased proliferation and migration of ADSCs, BM-MSCs, and HSCs, along with modulation of secreted proteins from those cells. Therefore, dHACM may impact wound healing by amplifying host stem cell populations and modulating their responses in treated wound tissues. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1495-1503, 2016.
机译：人源的胎盘组织已在随机临床试验中显示对治愈慢性伤口有效，并且还证明了在体外和体内将干细胞募集到伤口部位的能力。在这项研究中，评估了经过处理的脱水人羊膜/绒毛膜同种异体移植物（dHACM，EpiFix（R），MiMedx Group，Marietta，GA）在体外改变干细胞活性的能力。用dHACM组织的可溶性提取物处理人骨髓间充质干细胞（BM-MSC），脂肪衍生干细胞（ADSC）和造血干细胞（HSC），并评估其细胞增殖，迁移和细胞因子分泌。 24小时后通过DNA测定分析干细胞的细胞数，使用显微镜在3天内关闭无细胞区，并在3天后使用多重ELISA阵列分析在处理过的干细胞培养基中的可溶性细胞因子产生。用dHACM组织的可溶性提取物处理可刺激BM-MSC，ADSC和HSC增殖，并在24小时后细胞数量显着增加。与基础培养基相比，dHACM治疗在3天后加速了ADSC和BM-MSC对无细胞区的封闭。 BM-MSC，ADSC和HSC还可调节多种可溶性信号的内源性产生，包括炎症，有丝分裂和伤口愈合的调节剂。 dHACM处理促进了ADSC，BM-MSC和HSC的增殖和迁移以及这些细胞分泌蛋白的调节。因此，dHACM可通过扩增宿主干细胞群体并调节其在治疗伤口组织中的反应来影响伤口愈合。 （c）2015 Wiley Periodicals，Inc. J Biomed Mater Res B部分：Appl Biomater，104B：1495-1503，2016。

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