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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Electrochemical method for entrapment of oligonucleotides in polymer-coated electrodes
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Electrochemical method for entrapment of oligonucleotides in polymer-coated electrodes

机译：用于将寡核苷酸截留在聚合物涂层电极中的电化学方法

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摘要

Oligonucleotides were incorporated into conducting films of poly(3,4-ethylene dioxythiophene) by an electrochemical method that involves two steps. The electrode is first coated with the polymer film by electropoly-merization followed by electrooxidation of the formed polymer in the presence of the oligonucleotide. Neutral water soluble polymers such as poly(vinylpyrrolidone) or poly-(ethylene glycol) were added to the polymerizing medium resulting in higher incorporation yields of oligonucleotides. The results showed that a payload of about 1.5 nmol/cm~2 can be achieved in an 8 #mu#m thick film for an oligomer concentration of about 70 #mu#M in the working solution. Various physical methods were used to analyze the surface of the polymer-coated electrodes. Results showed the presence of domains of varying sizes at the film surface, corresponding to the insertion of the hydrophilic polymer within the matrix of the conductive polymer. The release of entrapped oligonucleotides from the coated film exhibited a three-step profile: a "burst" period during the first 5 min followed by an intermediate and a very slow release period lasting several days. Such polymer films could be exploited as useful reservoirs of biologically active substances for in vivo delivery to targeted tissues. For example, coated stents that can release antiproliferative agents such as antisense oligonucleotides at the site of balloon dilatation may be of interest in the treatment of postangioplasty restenosis.
机译：通过涉及两个步骤的电化学方法将寡核苷酸掺入到聚（3,4-乙撑二氧噻吩）的导电膜中。首先通过电聚合用聚合物膜涂覆电极，然后在寡核苷酸存在下将形成的聚合物电氧化。将中性水溶性聚合物，例如聚（乙烯基吡咯烷酮）或聚（乙二醇）添加到聚合介质中，从而导致寡核苷酸的掺入产率更高。结果表明，对于工作溶液中的低聚物浓度为约70＃μm，在8＃μm厚的膜中可获得约1.5nmol / cm 2的有效负载。各种物理方法被用来分析聚合物涂覆的电极的表面。结果显示在膜表面存在大小变化的畴，这对应于亲水性聚合物在导电聚合物基质内的插入。包被的寡核苷酸从涂膜的释放显示出三个步骤的过程：前5分钟为“爆发”期，随后为持续数天的中间和非常缓慢的释放期。这样的聚合物膜可以用作生物活性物质的有用的储库，以用于体内递送至靶组织。例如，可以在球囊扩张部位释放抗增殖剂如反义寡核苷酸的涂层支架在血管成形术后再狭窄的治疗中可能是有意义的。

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