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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes.
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Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes.

机译：壳聚糖支持人成骨细胞和软骨细胞中细胞外基质蛋白的表达。

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The search for biocompatible materials that can support the growth and phenotypic expression of osteoblasts and chondrocytes is a major challenge in the application of tissue engineering techniques for the repair of bone and cartilage defects. Chitosan, a copolymer of glucosamine and N-acetylglucosamine, may provide an answer to this search. Chitosan is the deacetylated product of chitin, a ubiquitous biopolymer found in the exoskeleton of insects and marine invertebrates. Little is known about the utility of chitosan in propagating human osteoblasts and chondrocytes. In this study, we test the hypothesis that chitosan promotes the survival and function of osteoblasts and chondrocytes. Chitosan (4%, w/v in 2% HAc) was coated onto plastic coverslips that had been fitted into 24-well plates. Human osteoblasts and articular chondrocytes were seeded on either uncoated or chitosan-coated coverslips at 1 x 10(5)/cells per well. Cultures were incubated at 37 degrees C, 5% CO(2) for a period of 7 days. Cell viability was assessed at that time using a fluorescent molecular probe. The phenotypic expression of osteoblasts and chondrocytes was analyzed by reverse transcriptase-polymerase chain reaction and immunocytochemistry. Osteoblasts and chondrocytes appeared spherical and refractile on chitosan-coated coverslips. In contrast, greater than 90% of cells on plastic coverslips were elongated and spindle shaped after 7 days of culture. Similar to cells propagated on uncoated control wells, greater than 90% of human osteoblasts and chondrocytes propagated on chitosan remained viable. Human osteoblasts propagated on chitosan films continued to express collagen type I whereas chondrocytes expressed collagen type II and aggrecan, as shown by reverse transcriptase-polymerase chain reaction analysis and immunostaining. The present in vitro work demonstrates the biocompatibility of chitosan as a substrate for the growth and continued function of human osteoblasts and chondrocytes. Chitosan may have potential use as a tissue engineering tool for the repair of osseous and chondral defects.
机译：寻找能够支持成骨细胞和软骨细胞生长和表型表达的生物相容性材料是组织工程技术在修复骨和软骨缺损中的一项重大挑战。壳聚糖是氨基葡萄糖和N-乙酰氨基葡萄糖的共聚物，可以为这一搜索提供答案。壳聚糖是几丁质的脱乙酰化产物，几丁质是在昆虫和海洋无脊椎动物外骨骼中普遍存在的生物聚合物。关于壳聚糖在繁殖人成骨细胞和软骨细胞中的效用知之甚少。在这项研究中，我们检验了壳聚糖促进成骨细胞和软骨细胞存活和功能的假说。将壳聚糖（4％，w / v在2％HAc中）包被到已安装在24孔板上的塑料盖玻片上。将人成骨细胞和关节软骨细胞以每孔1 x 10（5）/细胞的密度接种在未包被的或壳聚糖包被的盖玻片上。将培养物在37°C，5％CO（2）下孵育7天。当时使用荧光分子探针评估细胞活力。通过逆转录-聚合酶链反应和免疫细胞化学分析成骨细胞和软骨细胞的表型表达。成骨细胞和软骨细胞在壳聚糖涂层的盖玻片上呈球形并折射。相反，培养7天后，塑料盖玻片上超过90％的细胞被拉长并呈纺锤形。与在未包被的对照孔中繁殖的细胞相似，在壳聚糖上繁殖的人类成骨细胞和软骨细胞中，有90％以上仍保持活力。如逆转录酶-聚合酶链反应分析和免疫染色所示，在壳聚糖膜上繁殖的人类成骨细胞继续表达I型胶原，而软骨细胞表达II型胶原和聚集蛋白聚糖。目前的体外研究证明壳聚糖作为人类成骨细胞和软骨细胞的生长和持续功能的底物具有生物相容性。壳聚糖可能具有作为组织工程工具修复骨和软骨缺损的潜在用途。

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