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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Interaction of bioactive glasses with peritoneal macrophages and monocytes in vitro
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Interaction of bioactive glasses with peritoneal macrophages and monocytes in vitro

机译：生物活性玻璃与腹膜巨噬细胞和单核细胞的相互作用

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摘要

Macrophage activation was analyzed following exposure to pure, crystalline alpha-quartz powders, two bioactive gel-glass powders of different compositions, and a melt-derived glass, 45S5 Bioglass~R. The release of reactive oxygen metabolites (chemiluminescence test), modifications of cell morphology, the amount of tumor necrosis factor alpha (TNF alpha) secreted, and the amount of TNF alpha mRNA expression were evaluated. The 45S5 Bioglass~R powders elicited the highest chemiluminescence response while the two sol-gel glasses had a lower response with less of an oxidative burst difference between them. Particulate bioactive glasses are actively ingested by mouse peritoneal macrophages, and only the 58S sol-gel glass had a moderate toxic effect on the macrophages. Macrophage cell morphology showed increased size and cell spreading, consistent with the high level of cytokine secretion induced by 45S5 Bioglass~R. The 45S5 Bioglass~R powders led to an increased release of TNF alpha and expression of TNF alpha mRNA relative to unstimulated and control treated monocytes. Bioactive glasses (and particularly 45S5 Bioglass~R) that in vivo induce rapid bone growth appear to activate an autocrine-like process in which the response evoked by the material (for example monocyte and macrophage activation with cytokine production) enhances subsequent interactions with cells in contact with the material.
机译：暴露于纯净的结晶性α-石英粉末，两种不同组成的生物活性凝胶-玻璃粉末以及熔融衍生的玻璃45S5Bioglass®R后，分析巨噬细胞的活化。评价了活性氧代谢物的释放（化学发光测试），细胞形态的改变，肿瘤坏死因子α（TNFα）的分泌量以及TNFαmRNA的表达量。 45S5 Bioglass〜R粉末引起的化学发光反应最高，而两款溶胶凝胶玻璃的反应性较低，且两者之间的氧化猝灭差异较小。小鼠腹膜巨噬细胞会积极摄入颗粒状的生物活性玻璃，只有58S溶胶-凝胶玻璃对巨噬细胞具有中等毒性。巨噬细胞形态学表现为大小增加和细胞扩散，这与45S5 Bioglass〜R诱导的高水平细胞因子分泌相一致。相对于未刺激和对照处理的单核细胞，45S5 Bioglass〜R粉末导致TNFα释放增加和TNFαmRNA表达增加。体内诱导骨骼快速生长的生物活性玻璃（尤其是45S5Bioglass®R）似乎激活了自分泌样过程，其中物质引起的反应（例如单核细胞和巨噬细胞的激活与细胞因子的产生）增强了与细胞内随后的相互作用。与物料接触。

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