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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels
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Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels

机译：水凝胶特性影响光包裹在聚乙二醇水凝胶中的软骨细胞产生的ECM

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摘要

When using hydrogel scaffolds for cartilage tissue engineering, two gel properties are particularly important: the equilibrium water content (q, equilibrium swelling ratio) and the compressive modulus, K. In this work, chondrocytes were photoencapsulated in degrading and nondegrading poly(ethylene glycol)-based hydrogels to assess extracellular matrix (ECM) formation as a function of these gel properties. In nondegrading gels, the glycosaminoglycan (GAG) content was not significantly different in gels when q was varied from 4.2 to 9.3 after 2 and 4 weeks in vitro. However, gels with a q of 9.3 allowed GAGs to diffuse throughout the gels homogenously, but a q <= 5.2 resulted in localization of GAGs pericellularly. Interestingly, in the moderately crosslinked gels with a K of 360 kPa, an increase in type II collagen synthesis was observed compared with gels with a higher (960 kPa) and lower (30 kPa) K after 4 weeks. With the incorporation of degradable linkages into the network, gel properties with an initially high K (350 kPa) and final high q (7.9) were obtained, which allowed for increased type II collagen synthesis coupled with a homogenous distribution of GAGs. Thus, a critical balance exists between gel swelling, mechanics, and degradation in forming a functional ECM.
机译：当使用水凝胶支架进行软骨组织工程时，两个凝胶特性尤为重要：平衡水含量（q，平衡溶胀比）和压缩模量K。在这项工作中，软骨细胞被光囊化在降解和不可降解的聚乙二醇中基水凝胶来评估细胞外基质（ECM）的形成与这些凝胶特性的关系。在非降解凝胶中，当体外2周和4周后q从4.2变为9.3时，凝胶中的糖胺聚糖（GAG）含量无显着差异。但是，q为9.3的凝胶允许GAGs均匀扩散到整个凝胶中，但是q <= 5.2导致GAGs在细胞周围定位。有趣的是，在4周后，与K值较高（960 kPa）和较低（30 kPa）的凝胶相比，在K为360 kPa的中度交联凝胶中，观察到II型胶原蛋白合成增加。通过将可降解的键结合到网络中，获得了具有最初的高K（350 kPa）和最终的高q（7.9）的凝胶特性，这使得增加的II型胶原蛋白合成和GAG的均匀分布成为可能。因此，在形成功能性ECM的凝胶溶胀，力学和降解之间存在关键的平衡。

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