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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Effects of DMAEMA and 4-methoxyphenol on gingival fibroblast growth, metabolism, and response to interleukin-1
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Effects of DMAEMA and 4-methoxyphenol on gingival fibroblast growth, metabolism, and response to interleukin-1

机译：DMAEMA和4-甲氧基苯酚对牙龈成纤维细胞生长，代谢和对白介素-1的影响

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摘要

Some components of resins used in restorative dentistry have been shown to alter metabolism in cultured oral epithelial cells. Here we have extended such studies to the underlying supportive tissue, composed of gingival fibroblasts (CF). Primary cultures of human CF were transferred to serum-free, defined medium and exposed to either 2-(dimethylamino)ethyl methacrylate (DMAEMA) or 4-me-thoxyphenol (MEHQ) for 24-72 h. At a DMAEMA concentration of 6.4 mM, which was well tolerated by epithelial cells, CF numbers, as estimated by crystal violet, and metabolic activity, as indicated by MTT, were reduced at least 60 percent within 24 h of exposure. Between 1.6 and 6.4 mM, there were dose-related reductions in cell numbers; however, at lower doses (0.32-0.64 mM), proliferation was stimulated. MEHQ, between 8 and 16 mu M, did not stimulate cellular protein production. To examine the capacity of CF to respond to an inflammatory stimulus, interleukin-6 (IL-6) production by confluent cells was estimated without or with these compounds. DMAFMA (1.6-6.4 mM) virtually eliminated the acute IL-6 response of these cells to an interleukin-1 beta challenge; only at 0.32 mM DMAEMA was the response restored. MEHQ (1.6-16 mu M) reduced the IL-6 response by >50 percent. In summary, both growth and the innate immune responsivity of CF were affected by DMAEMA and MEHQ in vitro; thus, these compounds deserve careful evaluation for biocompatibility.
机译：修复性牙科中使用的树脂的某些成分已显示会改变培养的口腔上皮细胞的代谢。在这里，我们将此类研究扩展到由牙龈成纤维细胞（CF）组成的基础支持组织。将人CF的原代培养物转移至无血清的特定培养基中，然后将其暴露于甲基丙烯酸2-（二甲氨基）乙酯（DMAEMA）或4-甲氧基苯酚（MEHQ）中24-72小时。在上皮细胞良好耐受的DMAEMA浓度为6.4 mM的情况下，暴露24小时内，结晶紫所估计的CF值和MTT所表明的代谢活性至少降低了60％。在1.6和6.4 mM之间，与剂量相关的细胞数量减少;但是，在较低剂量（0.32-0.64 mM）下，会刺激增殖。 8至16μM的MEHQ不会刺激细胞蛋白质的产生。为了检查CF对炎症刺激的反应能力，估计在没有或没有这些化合物的情况下，融合细胞产生的白介素6（IL-6）产生量。 DMAFMA（1.6-6.4 mM）实际上消除了这些细胞对白介素1β激发的急性IL-6反应。仅在0.32 mM DMAEMA下恢复了响应。 MEHQ（1.6-16μM）使IL-6反应降低了50％以上。总之，体外DMAEMA和MEHQ影响CF的生长和先天免疫反应。因此，这些化合物的生物相容性值得仔细评估。

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