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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro engineering of human skin-like tissue.
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In vitro engineering of human skin-like tissue.

机译：人体皮肤样组织的体外工程。

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Coverage of large, full-thickness burns presents a challenge for the surgeon due to the lack of availability of the patient's own skin. Currently, tissue engineering offers the possibility of performing a suitable therapeutic wound coverage after early burn excision by using cultured keratinocyte sheets supported by a dermal layer. The aim of this study was to develop and characterize a skin substitute composed of both epidermal and dermal elements. For this purpose we grew keratinocytes and fibroblasts separately for 15 days within two different types of biomaterials. Cells then were co-cultured for an additional period of 15 days, after which samples were taken and processed with either classic or immunohistochemical stainings. Results showed that (1) human fibroblasts and keratinocytes can be cultured on hyaluronic acid-derived biomaterials and that (2) the pattern of expression of particular dermal-epidermal molecules is similar to that found in normal skin. The data from this study suggest that our skin equivalent might be useful in the treatment of both burns and chronic wounds.
机译：由于缺乏患者自己的皮肤，大而全层的烧伤覆盖范围给外科医生提出了挑战。当前，组织工程学提供了通过使用由真皮层支撑的培养的角质形成细胞片在早期烧伤切除后进行合适的治疗性伤口覆盖的可能性。这项研究的目的是开发和表征由表皮和真皮成分组成的皮肤替代品。为此，我们在两种不同类型的生物材料中分别使角质形成细胞和成纤维细胞生长了15天。然后将细胞共培养另外15天，然后取出样品并用经典或免疫组织化学染色进行处理。结果表明（1）可以在透明质酸衍生的生物材料上培养人成纤维细胞和角质形成细胞，并且（2）特定表皮-表皮分子的表达模式与正常皮肤相似。这项研究的数据表明，我们的皮肤等效物可能对烧伤和慢性伤口的治疗有用。

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