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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cartilage formation by fetal rat chondrocytes cultured in alginate beads: a proposed model for investigating tissue-biomaterial interactions.
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Cartilage formation by fetal rat chondrocytes cultured in alginate beads: a proposed model for investigating tissue-biomaterial interactions.

机译：由藻酸盐珠培养的胎儿大鼠软骨细胞形成的软骨：用于研究组织生物材料相互作用的拟议模型。

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摘要

Chondrocytes from 21-day-old rat fetal nasal cartilage were cultured in alginate beads for up to 20 days. It was found that chondrocytes retained their spherical shape and typical chondrocytic appearance. During the culture time, chondrocytes underwent differentiation, as demonstrated by the alkaline phosphatase-specific activity and rate of proteoglycan synthesis. Morphological data confirmed chondrocyte differentiation with the appearance of hypertrophic chondrocytes scattered in the alginate gel and a dense extracellular matrix containing filamentous structures and matrix vesicles. In addition, Northern blot analysis performed on day 8 of culture showed that chondrocytes cultured in alginate beads expressed type II collagen mRNA. The alginate bead method also appeared to be suitable for testing biomaterials, and the ready dissolution of the alginate beads by chelating agents provided a simple means for the rapid recovery of encapsulated chondrocytes. Powdered glass-ceramic particles entrapped in the alginate gel were colonized by chondrocytes, which then proliferated and formed a tissue similar to a true calcified cartilaginous structure. These results indicate that the alginate system represents a relevant model for studies of chondrogenesis and endochondral ossification. Furthermore, the encapsulation method could prove useful for studies of tissue-biomaterial interactions in an in vitro environment which more closely mirrors the cartilage matrix than other culture methods.
机译：将来自21天大的大鼠胎儿鼻软骨的软骨细胞在藻酸盐珠中培养最多20天。发现软骨细胞保持其球形和典型的软骨细胞外观。在培养期间，软骨细胞经历分化，这由碱性磷酸酶特异性活性和蛋白聚糖合成速率证明。形态学数据证实了软骨细胞的分化，肥大的软骨细胞散布在藻酸盐凝胶中，并且密集的细胞外基质含有丝状结构和基质囊泡。另外，在培养的第8天进行的RNA印迹分析表明，在藻酸盐珠中培养的软骨细胞表达II型胶原mRNA。藻酸盐珠的方法似乎也适用于测试生物材料，并且通过螯合剂使藻酸盐珠易于溶解提供了一种快速回收封装的软骨细胞的简单方法。藻酸盐凝胶中截留的粉末状玻璃陶瓷颗粒被软骨细胞定殖，然后增殖并形成类似于真实钙化软骨结构的组织。这些结果表明，藻酸盐系统代表了研究软骨形成和软骨内骨化的相关模型。此外，该封装方法可证明对体外环境中组织-生物材料相互作用的研究有用，该环境比其他培养方法更紧密地反映了软骨基质。

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