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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Biomimetic surface modification of poly (L-lactic acid) with gelatin and its effects on articular chondrocytes in vitro
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Biomimetic surface modification of poly (L-lactic acid) with gelatin and its effects on articular chondrocytes in vitro

机译：明胶对聚乳酸的仿生表面修饰及其对关节软骨细胞的影响

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Our objective in this study was to investigate the efficiency of two treatments for poly (L-lactic acid) (PLLA) surface modification with gelatin, via entrapment and coupling, using l-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The properties of original PLLA, gelatin-entrapped, and coupled PLLA films were investigated by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). The water contact angle indicated that the incorporation of gelatin resulted in a change in hydrophilicity, and the ESCA data suggested that the modified PLLA films became enriched with nitrogen atoms. The cytocompatibility of modified PLLA films might be improved. Therefore, we examined the attachment and proliferation of bovine articular chondrocyte seeded on modified PLLA films and virgin films. A whole-cell enzyme-linked immunosorbent assay (cell ELISA) that detects 5-bromo-2'-deoxyuridine (BrdU) incorporation during DNA synthesis and collagen type II secretion was applied to evaluate the chondrocytes on different PLLA films and tissue culture plates (TCPS). Cell viability was estimated by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay, and cell function was assessed by measuring glycosaminoglycan (GAG) secreted by chondrocytes. These results implied that gelatin used to modify the PLLA surface through entrapment and coupling could enhance chondrocyte adhesion, proliferation, and function.
机译：我们在这项研究中的目的是研究使用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）通过包封和偶联对明胶对聚L-乳酸（PLLA）表面进行改性的两种处理的效率和N-羟基琥珀酰亚胺（NHS）。通过水接触角测量和电子光谱进行化学分析（ESCA），研究了原始PLLA，明胶包裹和偶联PLLA膜的性能。水接触角表明明胶的掺入导致亲水性的改变，并且ESCA数据表明改性的PLLA膜变得富含氮原子。修饰的PLLA膜的细胞相容性可能得到改善。因此，我们检查了播种在改良的PLLA膜和原始膜上的牛关节软骨细胞的附着和增殖。应用全细胞酶联免疫吸附测定法（cell ELISA）检测DNA合成和II型胶原分泌过程中5-溴2'-脱氧尿苷（BrdU）掺入，以评估不同PLLA膜和组织培养板上的软骨细胞（ TCPS）。通过MTT [3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑鎓]测定法评估细胞活力，并通过测量软骨细胞分泌的糖胺聚糖（GAG）评估细胞功能。这些结果暗示明胶用于通过包封和偶联修饰PLLA表面可以增强软骨细胞的粘附，增殖和功能。

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