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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Field emission in-lens SEM study of enamel and dentin.
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Field emission in-lens SEM study of enamel and dentin.

机译:牙釉质和牙本质的透镜内场发射SEM研究。

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This investigation used field emission in-lens scanning electron microscopy (FEISEM) for the study of tooth surfaces, with particular reference to adhesive bonding and acid conditioning. Dentin wafers with an intact enamel periphery were treated by either ethylenediaminetetraacetic acid (EDTA) (pH 7.4) or phosphoric acid (pH 0.7). The samples were then fixed, sequentially dehydrated in alcohol, and either air- or critical point-dried. After coating, surfaces were examined by FEISEM. For enamel, intraprismatic crystals were clearly recognizable, with the crystals showing both a longitudinal and parallel orientation to the long axis of the prisms. For dentin, the surface ultrastructure (mineral crystals and collagen banding) for the both untreated and treated samples was observed. Fine structures measuring on the order of 6 nm were also observed on samples treated by EDTA. We conclude that FEISEM can routinely provide high-resolution images of enamel and dentin, and that it has the capability of revealing the defined distribution of crystals and collagen fibers in dental tissues. Copyright 1999 John Wiley & Sons, Inc.
机译:这项研究使用了场发射透镜内扫描电子显微镜(FEISEM)来研究牙齿表面,特别是对粘接和酸调节的研究。用乙二胺四乙酸(EDTA)(pH 7.4)或磷酸(pH 0.7)处理具有完整搪瓷外围的牙本质薄片。然后将样品固定,依次在酒精中脱水,然后风干或临界点干燥。涂覆后,通过FEISEM检查表面。对于搪瓷,可以清楚地辨认棱晶内晶体,该晶体相对于棱镜的长轴显示出纵向和平行方向。对于牙本质,观察到未处理和已处理样品的表面超微结构(矿物晶体和胶原蛋白条带)。在用EDTA处理的样品上也观察到了6nm量级的精细结构。我们得出的结论是,FEISEM可以常规提供牙釉质和牙本质的高分辨率图像,并且具有揭示牙齿组织中晶体和胶原纤维定义的分布的能力。版权所有1999 John Wiley&Sons,Inc.

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